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Sample GSM1110816 Query DataSets for GSM1110816
Status Public on Oct 20, 2013
Title GFI1_wildtype, rep2
Sample type RNA
 
Channel 1
Source name ILC2 cells, wildtype
Organism Mus musculus
Characteristics genotype: wildtype
cell type: ILC2
Growth protocol Cells were isolated from either wild-type or GFi1 KO mice. Purified ILC2s were cultured for 6 days in RPMI 1640, 10% FBS, Gln, P/S, 2ME, IL-2 (50ng/ml), IL-7 (10ng/ml), and IL-25 (50ng/ml).
Extracted molecule total RNA
Extraction protocol RNeasy Mini Kit (Qiagen).
Label Cy5
Label protocol Biotinylated cRNA were prepared according to the standard Agilent protocol.
 
Channel 2
Source name Stratagene Universal Mouse Reference RNA
Organism Mus musculus
Characteristics sample type: reference
Extracted molecule total RNA
Extraction protocol Stratagene Universal Mouse Reference RNA (catalog number 740100).
Label Cy3
Label protocol Biotinylated cRNA were prepared according to the standard Agilent protocol.
 
 
Hybridization protocol cRNA was hybridized on Agilent-026655 Whole Mouse Genome Microarray 4x44K.
Scan protocol Chips were scanned using the Agilent Surescan Microarray Scanner.
Description SAM9259610
Mouse wildtype ILC2 cells.
Data processing The data were processed with Agilent Feature Extraction software, version 10.7.3.1, using default settings, including linear and Lowess normalization.
 
Submission date Mar 28, 2013
Last update date Oct 20, 2013
Contact name Alex Abbas
E-mail(s) abbas@gene.com
Organization name Genentech, Inc.
Department Bioinformatics
Street address 1 DNA Way
City South San Francisco
State/province CA
ZIP/Postal code 94080
Country USA
 
Platform ID GPL11202
Series (1)
GSE45621 Mouse ILC2 cells from wild-type or Gfi1-KO animals

Data table header descriptions
ID_REF
VALUE Log2 ratio (ch1/ch2) representing test/reference

Data table
ID_REF VALUE
(+)E1A_r60_1
(+)E1A_r60_3
(+)E1A_r60_a104
(+)E1A_r60_a107
(+)E1A_r60_a135
(+)E1A_r60_a20
(+)E1A_r60_a22
(+)E1A_r60_a97
(+)E1A_r60_n11
(+)E1A_r60_n9
(-)3xSLv1
A_51_P100034 0.6137
A_51_P100174 0.9556
A_51_P100208 0.70372
A_51_P100289 0.076616
A_51_P100298 -2.4297
A_51_P100309 0.89655
A_51_P100327 2.9104
A_51_P100347 -0.74334
A_51_P100519

Total number of rows: 39485

Table truncated, full table size 800 Kbytes.




Supplementary file Size Download File type/resource
GSM1110816_Agilent_252665514661_S001_GE2_1100_Jul11_1_2.txt.gz 4.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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