RNA was isolated from peripheral blood collected in Tempus RNA tubes (Applied Biosystems, Darmstadt, Germany) at all three investigated time points for each woman in the discovery sample as well as during the third trimester only for the replication sample. RNA was isolated using the Versagene kit (Gentra Systems, Minneapolis, U.S.A.), quantified using the Nanophotometer and quality checks were performed on the Agilent Bioanalyzer.
Label
biotin
Label protocol
biotin
Hybridization protocol
Illumina protocol
Scan protocol
Illumina protocol
Description
Sample name: 103
Data processing
Microarray expression analysis was performed in R, using beadarray (providing routines to handle Illumina BeadStudio data) , limma (for statistical routines) and vsn (for normalization) packages. Raw microarray scan files were exported using the Illumina Beadstudio program and loaded into R for downstream analysis. The data were transformed and normalized using the variance stabilizing normalization method. Of the probes present on the Human HT-12v4, a total of 15456 probes passing the filter criteria of Illumina probe detection p-value of < 0.01 in at least 10% of the samples were used for subsequent analysis.
