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Sample GSM1104072 Query DataSets for GSM1104072
Status Public on May 27, 2013
Title sorted proB_wt_rep2
Sample type RNA
Source name sorted proB_wildtype mice_replicate2
Organism Mus musculus
Characteristics strain: C57BL/6
genotype/variation: Runx1 wildtype
cell type: proB-cells (CD19+/B220med/CD93+)
Treatment protocol ProB-cells (CD19+/B220med/CD93+) from either Runx1+/+Cd79ahCre/+ or Runx1fl/flCd79ahCre/+ mice were isolated.
Extracted molecule total RNA
Extraction protocol RNA was extracted (NucleoSpin® RNA II; Agilent Technologies), according to the manufacturer's instructions by Miltenyi Biotec.
Label Cy3
Label protocol Cy3-labeled cRNA was prepared using the Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies) following manufacturer´s protocol. The amount of cRNA and the dye-incorporation rate were checked by using the ND-1000 Spectrophotometer (NanoDrop Technologies) by Miltenyi Biotec.
Hybridization protocol Hybridization was peformed according to manufacturer´s protocol using the Agilent Gene Expression Hybridization Kit (Agilent Technologies). 1.65 µg Cy3-labeled fragmented cRNA was hybridized overnight (17 hrs., 65 °C) to Agilent Whole Mouse Genome Oligo Microarrays 4x44K by Miltenyi Biotec.
Scan protocol Fluorescence signals were scanned using the Agilent´s Microarray Scanner System (Agilent Technologies). The Agilent Feature Extraction Software (EFS) was used to read out and process the microarray image files.
Description Gene expression in proB cells of Runx1+/+Cd79ahCre/+ mice
Data processing The images were analyzed using the Agilent G2567AA Feature Extraction Software v.9.1.
Submission date Mar 22, 2013
Last update date May 28, 2013
Contact name Neele Margarete Kriebitzsch
Organization name Heinrich-Pette-Institute, Leibniz Institute for Experimental Virology
Lab Molecular Pathology
Street address Martinistraße 52
City Hamburg
ZIP/Postal code 20251
Country Germany
Platform ID GPL7202
Series (2)
GSE45424 Gene expression analysis to identify Runx1 target genes in B-cell progenitors
GSE45425 Runx1 targets in early B-cell progenitors

Data table header descriptions
VALUE Normalized signal intensity

Data table
A_51_P100021 1.498745
A_51_P100034 75.970556
A_51_P100052 0.033773
A_51_P100063 0.259766
A_51_P100084 1.04703
A_51_P100099 14.761441
A_51_P100155 57.286578
A_51_P100174 58.92269
A_51_P100181 10.96775
A_51_P100218 0.028922
A_51_P100227 35.862128
A_51_P100238 0.054475
A_51_P100246 45.221888
A_51_P100289 59.744848
A_51_P100298 0.258384
A_51_P100309 0.04178
A_51_P100327 50.782685
A_51_P100347 0.725199
A_51_P100379 1.450859
A_51_P100428 0.034469

Total number of rows: 41174

Table truncated, full table size 892 Kbytes.

Supplementary file Size Download File type/resource
GSM1104072_251486833325_S01_GE1_105_Jan09_1_3.txt.gz 9.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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