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Sample GSM1104071 Query DataSets for GSM1104071
Status Public on May 27, 2013
Title sorted proB_k.o._rep1
Sample type RNA
Source name sorted proB_knockout mice_replicate1
Organism Mus musculus
Characteristics strain: C57BL/6
genotype/variation: Runx1 knockout
cell type: proB-cells (CD19+/B220med/CD93+)
Treatment protocol ProB-cells (CD19+/B220med/CD93+) from either Runx1+/+Cd79ahCre/+ or Runx1fl/flCd79ahCre/+ mice were isolated.
Extracted molecule total RNA
Extraction protocol RNA was extracted (NucleoSpin® RNA II; Agilent Technologies), according to the manufacturer's instructions by Miltenyi Biotec.
Label Cy3
Label protocol Cy3-labeled cRNA was prepared using the Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies) following manufacturer´s protocol. The amount of cRNA and the dye-incorporation rate were checked by using the ND-1000 Spectrophotometer (NanoDrop Technologies) by Miltenyi Biotec.
Hybridization protocol Hybridization was peformed according to manufacturer´s protocol using the Agilent Gene Expression Hybridization Kit (Agilent Technologies). 1.65 µg Cy3-labeled fragmented cRNA was hybridized overnight (17 hrs., 65 °C) to Agilent Whole Mouse Genome Oligo Microarrays 4x44K by Miltenyi Biotec.
Scan protocol Fluorescence signals were scanned using the Agilent´s Microarray Scanner System (Agilent Technologies). The Agilent Feature Extraction Software (EFS) was used to read out and process the microarray image files.
Description Gene expression in proB cells of Runx1fl/flCd79ahCre/+ mice
Data processing The images were analyzed using the Agilent G2567AA Feature Extraction Software v.9.1.
Submission date Mar 22, 2013
Last update date May 28, 2013
Contact name Neele Margarete Kriebitzsch
Organization name Heinrich-Pette-Institute, Leibniz Institute for Experimental Virology
Lab Molecular Pathology
Street address Martinistraße 52
City Hamburg
ZIP/Postal code 20251
Country Germany
Platform ID GPL7202
Series (2)
GSE45424 Gene expression analysis to identify Runx1 target genes in B-cell progenitors
GSE45425 Runx1 targets in early B-cell progenitors

Data table header descriptions
VALUE Normalized signal intensity

Data table
A_51_P100021 1.183234
A_51_P100034 44.903373
A_51_P100052 0.034691
A_51_P100063 0.324993
A_51_P100084 1.177121
A_51_P100099 19.262151
A_51_P100155 62.92463
A_51_P100174 129.391216
A_51_P100181 15.550194
A_51_P100218 0.028527
A_51_P100227 39.386494
A_51_P100238 0.036163
A_51_P100246 46.378941
A_51_P100289 53.5318
A_51_P100298 0.204208
A_51_P100309 0.041599
A_51_P100327 74.438946
A_51_P100347 0.488689
A_51_P100379 2.261057
A_51_P100428 0.035934

Total number of rows: 41174

Table truncated, full table size 892 Kbytes.

Supplementary file Size Download File type/resource
GSM1104071_251486833325_S01_GE1_105_Jan09_1_2.txt.gz 9.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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