|Public on May 27, 2013
|sorted proB_wildtype mice_replicate1
genotype/variation: Runx1 wildtype
cell type: proB-cells (CD19+/B220med/CD93+)
|ProB-cells (CD19+/B220med/CD93+) from either Runx1+/+Cd79ahCre/+ or Runx1fl/flCd79ahCre/+ mice were isolated.
|RNA was extracted (NucleoSpin® RNA II; Agilent Technologies), according to the manufacturer's instructions by Miltenyi Biotec.
|Cy3-labeled cRNA was prepared using the Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies) following manufacturer´s protocol. The amount of cRNA and the dye-incorporation rate were checked by using the ND-1000 Spectrophotometer (NanoDrop Technologies) by Miltenyi Biotec.
|Hybridization was peformed according to manufacturer´s protocol using the Agilent Gene Expression Hybridization Kit (Agilent Technologies). 1.65 µg Cy3-labeled fragmented cRNA was hybridized overnight (17 hrs., 65 °C) to Agilent Whole Mouse Genome Oligo Microarrays 4x44K by Miltenyi Biotec.
|Fluorescence signals were scanned using the Agilent´s Microarray Scanner System (Agilent Technologies). The Agilent Feature Extraction Software (EFS) was used to read out and process the microarray image files.
|Gene expression in proB cells of Runx1+/+Cd79ahCre/+ mice
|The images were analyzed using the Agilent G2567AA Feature Extraction Software v.9.1.
|Mar 22, 2013
|Last update date
|May 28, 2013
|Neele Margarete Kriebitzsch
|Heinrich-Pette-Institute, Leibniz Institute for Experimental Virology
|Gene expression analysis to identify Runx1 target genes in B-cell progenitors
|Runx1 targets in early B-cell progenitors