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Sample GSM1104068 Query DataSets for GSM1104068
Status Public on May 27, 2013
Title proB-cell line_ERt2_EtOH
Sample type RNA
Source name cell line BMiFLT3(15-3)
Organism Mus musculus
Characteristics cell line: BMiFLT3(15-3)
cell type: proB cells from a murine BCP-ALL
genetic modification: cell transduced with ERt2
treatment: control
Treatment protocol 24 hours before sorting the cells were either treated with 0.2 µM OHT to ensure Runx1-ERt2 translocation into the nucleus or treated with the appropriate amount of ethanol as a control. For FACS-sort cells were stained with an antibody against B220. B220 positive cells were isolated and cell pellets were frozen at -80 °C until further processing steps were performed.
Growth protocol Cells were routinely grown in α-MEM with 10% FCS, glutamine and sodium pyruvate.
Extracted molecule total RNA
Extraction protocol RNA was extracted (NucleoSpin® RNA II; Agilent Technologies), according to the manufacturer's instructions by Miltenyi Biotec.
Label Cy3
Label protocol Cy3-labeled cRNA was prepared using the Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies) following manufacturer´s protocol. The amount of cRNA and the dye-incorporation rate were checked by using the ND-1000 Spectrophotometer (NanoDrop Technologies) by Miltenyi Biotec.
Hybridization protocol Hybridization was peformed according to manufacturer´s protocol using the Agilent Gene Expression Hybridization Kit (Agilent Technologies). 1.65 µg Cy3-labeled fragmented cRNA was hybridized overnight (17 hrs., 65 °C) to Agilent Whole Mouse Genome Oligo Microarrays 4x44K by Miltenyi Biotec.
Scan protocol Fluorescence signals were scanned using the Agilent´s Microarray Scanner System (Agilent Technologies). The Agilent Feature Extraction Software (EFS) was used to read out and process the microarray image files.
Data processing The images were analyzed using the Agilent G2567AA Feature Extraction Software v.9.1.
Submission date Mar 22, 2013
Last update date May 28, 2013
Contact name Neele Margarete Kriebitzsch
Organization name Heinrich-Pette-Institute, Leibniz Institute for Experimental Virology
Lab Molecular Pathology
Street address Martinistraße 52
City Hamburg
ZIP/Postal code 20251
Country Germany
Platform ID GPL11202
Series (2)
GSE45424 Gene expression analysis to identify Runx1 target genes in B-cell progenitors
GSE45425 Runx1 targets in early B-cell progenitors

Data table header descriptions
VALUE Normalized signal intensity

Data table
A_51_P100034 53.295954
A_51_P100174 75.890617
A_51_P100208 0.156954
A_51_P100289 50.33043
A_51_P100298 0.150276
A_51_P100309 0.130689
A_51_P100327 24.991791
A_51_P100347 1.660563
A_51_P100519 0.140854
A_51_P100537 0.155712
A_51_P100573 10.884088
A_51_P100624 0.135204
A_51_P100625 1.535043
A_51_P100768 1.022846
A_51_P100776 2.902431
A_51_P100787 111.206359
A_51_P100828 122.024422
A_51_P100852 5.107002
A_51_P100991 0.921135
A_51_P100997 8.043286

Total number of rows: 39429

Table truncated, full table size 884 Kbytes.

Supplementary file Size Download File type/resource
GSM1104068_252665512013_S01_GE1_105_Jan09_1_3.txt.gz 9.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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