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Sample GSM1103358 Query DataSets for GSM1103358
Status Public on Mar 22, 2013
Title InVivo_KO_1
Sample type RNA
Source name Mature FL-derived megakaryocytes, Runx1 KO
Organism Mus musculus
Characteristics strain/background: C57BL
genotype: Runx1 knockout
cell type: mature megakaryocytes
Growth protocol FL cells were derived from E14.5 embryos and cultured in DMEM medium supplemented with 10% FBS (Gibco, US), 2mM L-glutamine and penicillin/streptomycin and TPO (50ng ml-1) at 37ÂșC and 10% CO2. After 7 days in culture, mature MKs were isolated by two sequential 2%-4% BSA gradients.
Extracted molecule total RNA
Extraction protocol RNA was isolated using EZ-RNA (Biological Industries, Beit Haemek, Israel) according to the manufacturer's instructions.
Label biotin
Label protocol Purified RNA was reverse-transcribed, amplified and labeled with the Affymetrix GeneChip Whole Transcript Sense Target Labeling kit.
Hybridization protocol Samples were hybridized using Affymetrix hybridization kit materials.
Scan protocol Microarrays were scanned using GeneChip scanner 3000 7G.
Description YG151_MoST
Mature megakaryocytes derived from E14.5 fetal liver and cultured with TPO for 7 days.
Data processing Data was normalized using dChip.
Submission date Mar 21, 2013
Last update date Jul 30, 2013
Contact name Ram Jaschek
Organization name Weizmann Institute of Science
Street address P.O Box 26
City Rechovot
ZIP/Postal code 71600
Country Israel
Platform ID GPL6246
Series (2)
GSE45373 Cell-autonomous function of Runx1 transcriptionally regulates megakaryocytic maturation in mice (expression)
GSE45374 Cell-autonomous function of Runx1 transcriptionally regulates megakaryocytic maturation in mice

Data table header descriptions
VALUE log2-transformed dChip signal intensity

Data table
10338001 13
10338003 10.92
10338004 10.31
10338017 13.25
10338025 9.81
10338026 13.79
10338029 10.91
10338035 9.96
10338036 9.87
10338037 6.82
10338041 11.18
10338042 10.57
10338044 12.88
10338047 10.11
10338056 5.02
10338059 13.82
10338060 8
10338063 5.26
10338064 9.77
10338065 10.21

Total number of rows: 35512

Table truncated, full table size 484 Kbytes.

Supplementary file Size Download File type/resource
GSM1103358_YG151_MoST.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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