|
| Status |
Public on Mar 22, 2013 |
| Title |
InVivo_KO_1 |
| Sample type |
RNA |
| |
|
| Source name |
Mature FL-derived megakaryocytes, Runx1 KO
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL
genotype: Runx1 knockout
cell type: mature megakaryocytes
|
| Growth protocol |
FL cells were derived from E14.5 embryos and cultured in DMEM medium supplemented with 10% FBS (Gibco, US), 2mM L-glutamine and penicillin/streptomycin and TPO (50ng ml-1) at 37ºC and 10% CO2. After 7 days in culture, mature MKs were isolated by two sequential 2%-4% BSA gradients.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using EZ-RNA (Biological Industries, Beit Haemek, Israel) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Purified RNA was reverse-transcribed, amplified and labeled with the Affymetrix GeneChip Whole Transcript Sense Target Labeling kit.
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials.
|
| Scan protocol |
Microarrays were scanned using GeneChip scanner 3000 7G.
|
| Description |
YG151_MoST
Mature megakaryocytes derived from E14.5 fetal liver and cultured with TPO for 7 days.
|
| Data processing |
Data was normalized using dChip.
|
| |
|
| Submission date |
Mar 21, 2013 |
| Last update date |
Jul 30, 2013 |
| Contact name |
Ram Jaschek |
| E-mail(s) |
ramijaschek@gmail.com
|
| Organization name |
Weizmann Institute of Science
|
| Street address |
P.O Box 26
|
| City |
Rechovot |
| ZIP/Postal code |
71600 |
| Country |
Israel |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE45373 |
Cell-autonomous function of Runx1 transcriptionally regulates megakaryocytic maturation in mice (expression) |
| GSE45374 |
Cell-autonomous function of Runx1 transcriptionally regulates megakaryocytic maturation in mice |
|
