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Sample GSM1102810 Query DataSets for GSM1102810
Status Public on Mar 21, 2013
Title MVL1
Sample type RNA
 
Source name Left heart ventricle
Organism Mus musculus
Characteristics strain: C57Bl/6j
gender: male
develomental stage: adult
tissue: left heart ventricle
Treatment protocol heart was removed from adult male mice and each ventricle was isolated and minced
Extracted molecule total RNA
Extraction protocol Total RNA extraction was performed using RNAEasy Minikit (Qiagen, Germantown, MD, USA), following manufacturer's instructions. RNA concentration was determined with a Thermo Scientific NanoDrop ND-2000 and its quality with a 2100 Bioanalyzer (Agilent, DE)
Label Cy3
Label protocol Low Input Quick Amp labeling Kit two-color (Agilent, Cat # 5190-2306) that contains T7 RNA polymerase was used to simultaneously amplify total RNA and the positive controls (RNA Spike in Kit for Two Color, Agilent Cat # 5188-5279), and incorporate cyanine 3 (Cy3) or cyanine 5 (Cy5)-labeled CTP.
 
Hybridization protocol 825 ng from each of the Cy3 or Cy5 labeled samples from two biological replicas were mixed with Gene Expression Hybridization Kit (Agilent Cat # 5188-5242) and the final volume adjusted to 100 µL. The solution was slowly dispensed onto the gasket (Agilent Cat# G2534-60011) placed in the (Agilent) hybridization chamber, the (Agilent) mouse (4x44k 60 mer features, 4 microarrays per slide) chip with the active face downward was arranged over the gasket and the “sandwich” firmly closed. The samples were hybridized to the array at 65ºC for 17 h in an (Agilent) oven while vertically rotating (10 rot/min) to wet the gasket and asses the mobility of the bubbles.
Scan protocol The hybridized chip was washed at room temperature using the Gene Expression Wash Pack (Agilent, Cat# 5188-5327) and Stabilization and drying solution (Agilent, Cat# 5185-5979) then immediately scanned with an Agilent G2565CA microarray scanner system with SureScan high resolution technology at 5µm pixel size and 20-bit scan mode.
Description Gene expression in Left heart ventricle of adult male mouse
MVL_1-2.txt
raw data file:
WT_MV_L12_US83300186_252665512247_S01_GE2_1100_Jul11_1_2.txt
Data processing The resulted Tagged Image File Format (tiffs) obtained through use of (Agilent) Scan Control Software 8.3 were analyzed with (Agilent) Feature Extraction vs.11 software. Data were normalized and analyzed with in-house developed algorithms described in Iacobas et al., Mol Genet Genomics 2012.
normalized log2 ratio of the background subtracted signal of that feature and the median of the background subtracted signals of all valid features in the green or red channel of the array. A NULL indicates that the feature was not considered because is a control fetarure, OR is locally corrupted, OR has saturated pixels, OR the median of the foreground signal is less than 2x median of the background signal
 
Submission date Mar 20, 2013
Last update date Mar 21, 2013
Contact name Dumitru Andrei Iacobas
E-mail(s) daiacobas@pvamu.edu
Phone 936-261-9926
Organization name Prairie View A&M University
Department Center for Computational Systems Biology
Lab Personalized Genomics
Street address Ann Preston St
City Prairie View
State/province TX
ZIP/Postal code 77446
Country USA
 
Platform ID GPL10333
Series (1)
GSE45348 Left-right transcriptomic differences in adult male mouse heart ventricles

Data table header descriptions
ID_REF
VALUE normalized

Data table
ID_REF VALUE
1 null
2 null
3 null
4 null
5 null
6 null
7 null
8 null
9 null
10 null
11 null
12 6.460203916
13 0.646572846
14 3.56148289
15 1.264921515
16 3.850931345
17 5.40002999
18 1.268061223
19 1.292935892
20 1.370938404

Total number of rows: 44397

Table truncated, full table size 776 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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