|
| Status |
Public on Apr 11, 2013 |
| Title |
SVZ_NSCs_2 [lncRNA Array] |
| Sample type |
RNA |
| |
|
| Source name |
Mouse Brain_activated stem cells (NSCs)
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
cell type: FACS isolated SVZ stem cells
|
| Treatment protocol |
For FACS isolated cells, total SVZ was dissected and subjected to the FACS protocol detailed in Pastrana, et al 2009
|
| Growth protocol |
SVZ cells were grown in monolayer in proliferation media. At passage 5-7, cells were switched to media without growth factors for indicated timepoints (1,2,4 days)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with trizol reagent and amplified with Whole Transcriptome Amplification Kit (Thermo).
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed according to standard Nimblegen Protocols
|
| |
|
| Hybridization protocol |
Hybridization was perfomed on a Maui Hybridization System
|
| Scan protocol |
Scanning was done on a GenePix 4000B scanner according to standard Nimblegen protocols
|
| Description |
SAMPLE 2
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249) as implemented in the DEVA package, version 1.2.1
Sample data table contains RMA-normalized, averaged gene-level signal intensity (i.e. values in the 'EXPRS' column in processed data *RMA.calls file)
|
| |
|
| Submission date |
Mar 19, 2013 |
| Last update date |
Jan 01, 2019 |
| Contact name |
Abhinav Nellore |
| E-mail(s) |
abhinav.nellore@ucsf.edu
|
| Phone |
4439101925
|
| Organization name |
UCSF
|
| Street address |
513 Parnassus Avenue
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143-0794 |
| Country |
USA |
| |
|
| Platform ID |
GPL16745 |
| Series (2) |
| GSE45281 |
Integration of genome-wide approaches identifies lncRNAs of adult neural stem cells and their progeny in vivo [lncRNA Array] |
| GSE45282 |
Integration of genome-wide approaches identifies lncRNAs of adult neural stem cells and their progeny in vivo |
|
