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Sample GSM1100722 Query DataSets for GSM1100722
Status Public on Apr 11, 2013
Title SVZ_NSCs_1 [lncRNA Array]
Sample type RNA
Source name Mouse Brain_activated stem cells (NSCs)
Organism Mus musculus
Characteristics strain background: C57BL/6
cell type: FACS isolated SVZ stem cells
Treatment protocol For FACS isolated cells, total SVZ was dissected and subjected to the FACS protocol detailed in Pastrana, et al 2009
Growth protocol SVZ cells were grown in monolayer in proliferation media. At passage 5-7, cells were switched to media without growth factors for indicated timepoints (1,2,4 days)
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with trizol reagent and amplified with Whole Transcriptome Amplification Kit (Thermo).
Label Cy3
Label protocol Labeling was performed according to standard Nimblegen Protocols
Hybridization protocol Hybridization was perfomed on a Maui Hybridization System
Scan protocol Scanning was done on a GenePix 4000B scanner according to standard Nimblegen protocols
Description SAMPLE 1
Data processing The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249) as implemented in the DEVA package, version 1.2.1
Sample data table contains RMA-normalized, averaged gene-level signal intensity (i.e. values in the 'EXPRS' column in processed data *RMA.calls file)
Submission date Mar 19, 2013
Last update date Jan 01, 2019
Contact name Abhinav Nellore
Phone 4439101925
Organization name UCSF
Street address 513 Parnassus Avenue
City San Francisco
State/province CA
ZIP/Postal code 94143-0794
Country USA
Platform ID GPL16745
Series (2)
GSE45281 Integration of genome-wide approaches identifies lncRNAs of adult neural stem cells and their progeny in vivo [lncRNA Array]
GSE45282 Integration of genome-wide approaches identifies lncRNAs of adult neural stem cells and their progeny in vivo

Data table header descriptions
VALUE RMA-normalized, averaged gene-level signal intensity (EXPRS)

Data table
AK005028_CONTROL_SEQS 1349.5686
AK010953_CONTROL_SEQS 1874.5634
AK039117_CONTROL_SEQS 1115.3397
AK128971_CONTROL_SEQS 18374.6384
AK129421_CONTROL_SEQS 7812.9223
AK140151_CONTROL_SEQS 322.8192
AK143943_CONTROL_SEQS 83.0072
AY971958_CONTROL_SEQS 5903.4543
BC001994_CONTROL_SEQS 11311.2334
BC003772_CONTROL_SEQS 10251.3461
BC003833_CONTROL_SEQS 28097.0790
BC007135_CONTROL_SEQS 1942.0268
BC007466_CONTROL_SEQS 912.3156
BC011310_CONTROL_SEQS 35.7505
BC012966_CONTROL_SEQS 4834.5100
BC016421_CONTROL_SEQS 136.1257
BC023729_CONTROL_SEQS 6044.6883
BC023796_CONTROL_SEQS 6249.1951
BC025073_CONTROL_SEQS 1148.7340
BC028816_CONTROL_SEQS 15940.7226

Total number of rows: 17664

Table truncated, full table size 899 Kbytes.

Supplementary file Size Download File type/resource
GSM1100722_521989A01_custom_2012-04-04T154230_532.pair.gz 2.1 Mb (ftp)(http) PAIR
GSM1100722_521989A01_custom_532_RMA.calls.gz 252.9 Kb (ftp)(http) CALLS
Processed data included within Sample table
Processed data provided as supplementary file

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