|
| Status |
Public on Apr 11, 2013 |
| Title |
H3K27me3_ChIPSeq |
| Sample type |
SRA |
| |
|
| Source name |
subventricular zone neural stem cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: male
genotype/variation: WT
chip antibody: H3K27me3 antibody
chip antibody vendor: Millipore
chip antibody cat. #: 07-449
|
| Growth protocol |
SVZ was accutely isolated and cells were cultured as neurospheres on low-attachment plates
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.
Libraries were prepared using standard Illumina protocols
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Description |
Sample 2
|
| Data processing |
Reads aligned using Bowtie v1.1.2
Peaks called using MACs 1.0.1
Genome_build: mm9
Supplementary_files_format_and_content: .bed of called peaks
|
| |
|
| Submission date |
Mar 19, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Abhinav Nellore |
| E-mail(s) |
abhinav.nellore@ucsf.edu
|
| Phone |
4439101925
|
| Organization name |
UCSF
|
| Street address |
513 Parnassus Avenue
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143-0794 |
| Country |
USA |
| |
|
| Platform ID |
GPL11002 |
| Series (2) |
| GSE45279 |
Integration of genome-wide approaches identifies lncRNAs of adult neural stem cells and their progeny in vivo [ChIP-seq] |
| GSE45282 |
Integration of genome-wide approaches identifies lncRNAs of adult neural stem cells and their progeny in vivo |
|
| Relations |
| SRA |
SRX252184 |
| BioSample |
SAMN01984496 |
