|
| Status |
Public on Sep 01, 2013 |
| Title |
Myomet0101 [Expression] |
| Sample type |
RNA |
| |
|
| Source name |
Control
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
tissue: frozen tissue of myometrium without leiomyoma
disease state: normal myometrium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from tissues using the ISOGEN (NIPPON GENE)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared from 200 ng total RNA with the Ambion WT Expression Kit (Ambion) and Affymetrix GeneChip WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
control myometrium tissue to compare with myometrium with leiomyoma
|
| Data processing |
The data were analyzed with Partek Genomics Suite 6.5 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Mar 15, 2013 |
| Last update date |
Sep 01, 2013 |
| Contact name |
Ryo Maekawa |
| E-mail(s) |
ryomaekawa@gmail.com
|
| Organization name |
Yamaguchi University School of Medicine
|
| Department |
Department of Obstetrics and Gynecology
|
| Street address |
Minami-Kogushi 1-1-1
|
| City |
Ube |
| ZIP/Postal code |
755-8505 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE45188 |
Epigenome analysis of uterin leiomyoma and myometrium with or without leiomyoma [Expression data] |
| GSE45189 |
Epigenome analysis of uterin leiomyoma and myometrium with or without leiomyoma |
|
