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Sample GSM1098617 Query DataSets for GSM1098617
Status Public on May 01, 2013
Title E13.5PGCs_rep2
Sample type RNA
 
Source name PGCs derived from mouse E13.5 embryo genital ridges
Organism Mus musculus
Characteristics strain/background: MCH x C57BL6/J (Oct4ΔPE-GFP Tg)
gender: male
cell type: primordial germ cells (PGCs)
Treatment protocol The genital ridges were dissected from mouse E13.5 embryos. After trypsin treatment, live, single and GFP-positive cells were purified using FACS from the dissociated cells.
Extracted molecule total RNA
Extraction protocol Total RNA was purified using the RNeasy micro kit according to the manufacturer's instructions.
Label Cy3
Label protocol Cy3-labeled cRNA samples were prepared using the Agilent Low Input Quick Amp Labeling Kit (one-color) (5190-2305) and RNA Spike-In Kit (one-color) (5188-5282) according to the manufacturer's instructions. Input was 100 ng of total RNA from each sample.
 
Hybridization protocol 1.65ug of Cy3-labelled cRNAs from each sample were fragmented and hybridized to microarrays according to the manufacturer's instructions using the Gene Expression Hybridization kit (Agilent 5188-5242) and Agilent hybridization oven. After hybridization, microarrays were washed using the Gene Expression Wash Pack (Agilent 5188-5327).
Scan protocol The microarrays were scanned using the Agilent DNA microarray scanner (Agilent G2505C); the spot signals were quantified using Feature Extraction software.
Description Gene expression of PGCs from mouse E13.5 embryo genital ridges.
Data processing Data was normalized and analyzed by GeneSpring GX (Agilent).
 
Submission date Mar 14, 2013
Last update date May 01, 2013
Contact name Ikuma Maeda
E-mail(s) ikuma@idac.tohoku.ac.jp
Phone +81-22-717-8572
Fax +81-22-717-8573
Organization name Institute of Development, Aging and Cancer, Tohoku University
Lab Cell Resource Center for Biomedical Research
Street address 4-1 Seiryomachi, Aoba-ku
City Sendai
State/province Miyagi
ZIP/Postal code 980-8575
Country Japan
 
Platform ID GPL11202
Series (1)
GSE45181 Max is a repressor of germ-cell-related gene expression in mouse embryonic stem cells

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner 0
DarkCorner 0.6238694
A_55_P1989846 1.7427387
A_55_P1991598 1.3214579
A_55_P2022211 0
A_55_P1980764 -0.35791636
A_55_P1964375 0.30791473
A_51_P128876 0.1834774
A_55_P2121042 0.55347157
A_52_P219230 0.5557151
A_51_P207591 -3.1395721
A_55_P2131920 0.5605259
A_55_P2404223 0.78586006
A_55_P2101944 -1.2994652
A_52_P358860 0.22662258
A_51_P119031 0.08376026
A_51_P309854 -0.5219326
A_51_P343900 0.50909424
A_51_P234359 0.25157642
A_51_P487813 -1.0662184

Total number of rows: 39485

Table truncated, full table size 921 Kbytes.




Supplementary file Size Download File type/resource
GSM1098617_252665511681_1.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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