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Sample GSM1098611 Query DataSets for GSM1098611
Status Public on May 01, 2013
Title ESCs_Max_rep1
Sample type RNA
Source name VV3 ESCs, Max siRNA transfected
Organism Mus musculus
Characteristics strain/background: 129SV
gender: male
cell line: VV3
cell type: embryonic stem cells (ESCs)
sirna: Max
Treatment protocol ESCs were transfected with siRNA against the Max gene using Lipofectamine RNAiMAX. After 3 days culture, live, single and Vasa::Venus positive cells were purified using FACS from the ESCs.
Growth protocol Cells were cultured with ESCs medium with 14% KSR, 1% FBS and LIF at 37 ÂșC in a humidified incubator with 5% CO2.
Extracted molecule total RNA
Extraction protocol Total RNA was purified using the RNeasy micro kit according to the manufacturer's instructions.
Label Cy3
Label protocol Cy3-labeled cRNA samples were prepared using the Agilent Low Input Quick Amp Labeling Kit (one-color) (5190-2305) and RNA Spike-In Kit (one-color) (5188-5282) according to the manufacturer's instructions. Input was 100 ng of total RNA from each sample.
Hybridization protocol 1.65ug of Cy3-labelled cRNAs from each sample were fragmented and hybridized to microarrays according to the manufacturer's instructions using the Gene Expression Hybridization kit (Agilent 5188-5242) and Agilent hybridization oven. After hybridization, microarrays were washed using the Gene Expression Wash Pack (Agilent 5188-5327).
Scan protocol The microarrays were scanned using the Agilent DNA microarray scanner (Agilent G2505C); the spot signals were quantified using Feature Extraction software.
Description Gene expression of Max siRNA-transfected VV3 mouse ESCs.
Data processing Data was normalized and analyzed by GeneSpring GX (Agilent).
Submission date Mar 14, 2013
Last update date May 01, 2013
Contact name Ikuma Maeda
Phone +81-22-717-8572
Fax +81-22-717-8573
Organization name Institute of Development, Aging and Cancer, Tohoku University
Lab Cell Resource Center for Biomedical Research
Street address 4-1 Seiryomachi, Aoba-ku
City Sendai
State/province Miyagi
ZIP/Postal code 980-8575
Country Japan
Platform ID GPL11202
Series (1)
GSE45181 Max is a repressor of germ-cell-related gene expression in mouse embryonic stem cells

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner 0.14814281
DarkCorner -0.24087381
A_55_P1989846 -0.10918665
A_55_P1991598 -0.5416107
A_55_P2022211 0.32878113
A_55_P1980764 -0.22499561
A_55_P1964375 -0.25282574
A_51_P128876 -0.991025
A_55_P2121042 -0.24161291
A_52_P219230 -0.24180746
A_51_P207591 0.2883215
A_55_P2131920 -0.24217129
A_55_P2404223 -0.13097668
A_55_P2101944 0
A_52_P358860 -0.74464035
A_51_P119031 0.21885395
A_51_P309854 0.37987518
A_51_P343900 -0.45769215
A_51_P234359 -0.24007225
A_51_P487813 0.10548496

Total number of rows: 39485

Table truncated, full table size 927 Kbytes.

Supplementary file Size Download File type/resource
GSM1098611_252665511678_2.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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