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Sample GSM1087639 Query DataSets for GSM1087639
Status Public on Apr 01, 2013
Title SNDISH15, biological rep2
Sample type RNA
Source name SND1 knockdown 2
Organism Homo sapiens
Characteristics cell line: QGY-7703
cell type: human hepatocellular carcinoma
Treatment protocol No treatment was done.
Growth protocol Steady-state proliferating cells.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed using QIAGEN columns according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA (GeneChip 3' IVT Express Kit, P/N 702646 Rev. 8, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133A 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using protocol FS450_0002.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G.
Description SNDISH15_2
Gene expression data from SND1 knockdown 2.
Data processing The data were analyzed with Affymetrix Expression Console software using Affymetrix default analysis settings and RMA as the normalization method.
Submission date Feb 23, 2013
Last update date Apr 01, 2013
Contact name Myrna Serrano
Organization name Virginia Commonwealth University
Street address 1101 East Marshall St
City Richmond
State/province VA
ZIP/Postal code 23298
Country USA
Platform ID GPL571
Series (1)
GSE44601 Expression data from SND1 knockdown clones of human HCC cell line QGY-7703

Data table header descriptions
VALUE Log2 RMA signal

Data table
1007_s_at 10.13871
1053_at 8.417942
117_at 4.92451
121_at 7.434855
1255_g_at 2.7388
1294_at 5.28737
1316_at 6.009518
1320_at 4.12985
1405_i_at 4.343153
1431_at 4.203232
1438_at 5.373716
1487_at 8.617345
1494_f_at 5.246913
1598_g_at 7.993962
160020_at 6.202676
1729_at 7.484011
177_at 5.293616
1773_at 5.358155
179_at 7.984843
1861_at 6.527389

Total number of rows: 22277

Table truncated, full table size 431 Kbytes.

Supplementary file Size Download File type/resource
GSM1087639_SNDISH-15-2_HG-U133A_2_.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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