GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1087633 Query DataSets for GSM1087633
Status Public on Apr 01, 2013
Title QGY, biological rep2
Sample type RNA
Source name control clone
Organism Homo sapiens
Characteristics cell line: QGY-7703
cell type: human hepatocellular carcinoma
Treatment protocol No treatment was done.
Growth protocol Steady-state proliferating cells.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed using QIAGEN columns according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA (GeneChip 3' IVT Express Kit, P/N 702646 Rev. 8, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133A 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using protocol FS450_0002.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G.
Description QGY_2
Gene expression data from control clone.
Data processing The data were analyzed with Affymetrix Expression Console software using Affymetrix default analysis settings and RMA as the normalization method.
Submission date Feb 23, 2013
Last update date Apr 01, 2013
Contact name Myrna Serrano
Organization name Virginia Commonwealth University
Street address 1101 East Marshall St
City Richmond
State/province VA
ZIP/Postal code 23298
Country USA
Platform ID GPL571
Series (1)
GSE44601 Expression data from SND1 knockdown clones of human HCC cell line QGY-7703

Data table header descriptions
VALUE Log2 RMA signal

Data table
1007_s_at 9.737348
1053_at 8.840768
117_at 4.736669
121_at 7.252574
1255_g_at 2.772105
1294_at 5.055675
1316_at 5.969676
1320_at 4.153771
1405_i_at 3.413426
1431_at 3.960358
1438_at 5.190948
1487_at 8.61149
1494_f_at 5.062008
1598_g_at 7.572593
160020_at 5.793399
1729_at 7.455971
177_at 5.194395
1773_at 5.530314
179_at 7.708905
1861_at 6.883764

Total number of rows: 22277

Table truncated, full table size 431 Kbytes.

Supplementary file Size Download File type/resource
GSM1087633_Qgy2_HG-U133A_2_.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap