GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1087143 Query DataSets for GSM1087143
Status Public on Sep 02, 2015
Title E11.5 heart, Nkx2-5 ChIP (S1)
Sample type SRA
Source name E11.5 heart, Nkx2-5 ChIP
Organism Mus musculus
Characteristics strain: Parkes
developmental stage: E11.5
tissue: heart
Extracted molecule genomic DNA
Extraction protocol Fixed embryonic hearts were homogenised using an Ultra-Turrax, sonicated and Nkx2-5-DNA complexes were isolated using NKX-2.5 (N-19) sc-8697 antibody from Santa Cruz.
Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303).
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer IIx
Description S1
Data processing Basecalls performed using CASAVA version 1.4
ChIP-seq reads were aligned to the mm9 genome assembly using Bowtie.
peaks were called using MACS1.4, p-value=1x10-14
Genome_build: mm9
Supplementary_files_format_and_content: .bed files containing MACS peaks
Submission date Feb 22, 2013
Last update date May 15, 2019
Contact name Laurent Dupays
Organization name The Francis Crick Institute
Lab Mill Hill Laboratory
Street address The Ridgeway, Mill Hill
City London
ZIP/Postal code NW7 1AA
Country United Kingdom
Platform ID GPL11002
Series (2)
GSE44574 Genome-wide maps of Nkx2-5 in E11.5 mouse embryonic heart
GSE44576 Identification of NKX2-5 transcriptional targets in the mouse embryonic heart
SRA SRX244286
BioSample SAMN01924408

Supplementary file Size Download File type/resource
GSM1087143_nkx_s1e14_peaks.bed.gz 66.0 Kb (ftp)(http) BED
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap