NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1087143 Query DataSets for GSM1087143
Status Public on Sep 02, 2015
Title E11.5 heart, Nkx2-5 ChIP (S1)
Sample type SRA
 
Source name E11.5 heart, Nkx2-5 ChIP
Organism Mus musculus
Characteristics strain: Parkes
developmental stage: E11.5
tissue: heart
Extracted molecule genomic DNA
Extraction protocol Fixed embryonic hearts were homogenised using an Ultra-Turrax, sonicated and Nkx2-5-DNA complexes were isolated using NKX-2.5 (N-19) sc-8697 antibody from Santa Cruz.
Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303).
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer IIx
 
Description S1
Data processing Basecalls performed using CASAVA version 1.4
ChIP-seq reads were aligned to the mm9 genome assembly using Bowtie.
peaks were called using MACS1.4, p-value=1x10-14
Genome_build: mm9
Supplementary_files_format_and_content: .bed files containing MACS peaks
 
Submission date Feb 22, 2013
Last update date May 15, 2019
Contact name Laurent Dupays
E-mail(s) laurent.dupays@crick.ac.uk
Organization name The Francis Crick Institute
Lab Mill Hill Laboratory
Street address The Ridgeway, Mill Hill
City London
ZIP/Postal code NW7 1AA
Country United Kingdom
 
Platform ID GPL11002
Series (2)
GSE44574 Genome-wide maps of Nkx2-5 in E11.5 mouse embryonic heart
GSE44576 Identification of NKX2-5 transcriptional targets in the mouse embryonic heart
Relations
SRA SRX244286
BioSample SAMN01924408

Supplementary file Size Download File type/resource
GSM1087143_nkx_s1e14_peaks.bed.gz 66.0 Kb (ftp)(http) BED
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap