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Sample GSM1087142 Query DataSets for GSM1087142
Status Public on Sep 02, 2015
Title E11.5 heart, Input
Sample type SRA
Source name E11.5 heart, Input
Organism Mus musculus
Characteristics strain: Parkes
developmental stage: E11.5
tissue: heart
Extracted molecule genomic DNA
Extraction protocol Fixed embryonic hearts were homogenised using an Ultra-Turrax, sonicated and Nkx2-5-DNA complexes were isolated using NKX-2.5 (N-19) sc-8697 antibody from Santa Cruz.
Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303).
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer IIx
Description Input
Data processing Basecalls performed using CASAVA version 1.4
ChIP-seq reads were aligned to the mm9 genome assembly using Bowtie.
peaks were called using MACS1.4, p-value=1x10-14
Genome_build: mm9
Supplementary_files_format_and_content: .bed files containing MACS peaks
Submission date Feb 22, 2013
Last update date May 15, 2019
Contact name Laurent Dupays
Organization name The Francis Crick Institute
Lab Mill Hill Laboratory
Street address The Ridgeway, Mill Hill
City London
ZIP/Postal code NW7 1AA
Country United Kingdom
Platform ID GPL11002
Series (2)
GSE44574 Genome-wide maps of Nkx2-5 in E11.5 mouse embryonic heart
GSE44576 Identification of NKX2-5 transcriptional targets in the mouse embryonic heart
SRA SRX244285
BioSample SAMN01924407

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data not provided for this record

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