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Sample GSM1084834 Query DataSets for GSM1084834
Status Public on Sep 13, 2016
Title hESC (ESO3) p76
Sample type RNA
 
Source name ES03 human Embryonic Stem Cells, Passage 76 (ES Cell International)
Organism Homo sapiens
Characteristics cell type: ES03 human Embryonic Stem Cells, Passage 76 (ES Cell International)
passage: p76
sample type: Undifferentiated hESC
Extracted molecule total RNA
Extraction protocol Total RNA was prepared as described in the RNeasy Mini Kit (Qiagen) with on-column DNase I digestion. RNA quality was assessed by Nanodrop-1000 spectrometer for OD260/280 and OD260/230 ratio and Bioanalyzer (Agilent Technologies).
Label Cy3
Label protocol 500 ng total RNA from each sample was amplified and labeled using the Illumina TotalPrep RNA Amplification Kit (AMIL1791, Ambion, Austin, TX) as described in the instruction manual.
 
Hybridization protocol For array assay, 750 ng biotin-labeled cRNA was combined with hybridization buffer and hybridized to the array at 58°C for 16-20 hours. After hybridization, the hybridization cartridge was disassembled and the array was washed with buffer at 55C and blocked at room temperature. Bound biotinylated cRNA was stained with streptavidin-Cy3 and then washed. Illumina HumanHT-12 V3.0 expression beadchips were used for all analyses in this series.
Scan protocol Fluorescent signals were obtained by scanning with the iScan System.
Description Individual sample
Data processing Data were extracted with Gene Expression Module 1.0.6 in GenomeStudio 1.0.2 and signal intensities from multiple chips comprising this single series were quantile normalized without background subtraction to obtain normalized data as deposited.
 
Submission date Feb 20, 2013
Last update date Sep 13, 2016
Contact name Elias Thomas Zambidis
Organization name Johns Hopkins School of Medicine
Department Oncology
Street address 733 N. Broadway
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
 
Platform ID GPL16700
Series (2)
GSE44421 Global gene expression analysis of donor cells, low passage episomal stromal-primed myeloid iPSC, and intermediate passage fibroblast iPSC.
GSE44430 Comprehensive microarray analysis of reprogramming fidelity in a library of non-integrated induced pluripotent stem cell lines prepared with various derivation methods

Data table header descriptions
ID_REF
VALUE signal intensity

Data table
ID_REF VALUE
7A5 112.2
A1BG 106.4
A1CF 107.3
A26C3 122.2
A2BP1 112.2
A2M 119.5
A2ML1 141
A3GALT2 111.8
A4GALT 134.2
A4GNT 99.3
AAA1 108
AAAS 226.2
AACS 465.7
AACSL 112.6
AADAC 109.3
AADACL1 136.4
AADACL2 100.1
AADACL3 106.1
AADACL4 113
AADAT 188

Total number of rows: 37867

Table truncated, full table size 514 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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