|
| Status |
Public on Jun 18, 2013 |
| Title |
ms1 unpollinated pistils, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Arabidopsis thaliana unpollinated ms1 (Male Sterile 1) pistils at stage 14
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: pistil
genotype: ms1 (Male sterile 1), Ler-0 Ecotype
age: Stage 14 Pistils
protocol: unpollinated
|
| Treatment protocol |
Pistils were dissected from surrounding floral tissues and immerdiately frozen on dry ice
|
| Growth protocol |
ms1 pistils (100 per collection) were either collected at stage 14 without pollination, or pollinated at 9am with respective genotypes and allowed to grow for 8 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy kit (Qiagen), The yield and RNA purity were determined by Nano-Drop (Thermo Scientific), and RNA integrity was checked using an Agilent 2100 Bioanalyzer (Agilent Technologies)
|
| Label |
biotin
|
| Label protocol |
100 ng total RNA was amplified using Affymetrix 3' IVT express kit according to manufacturers instructions.
|
| |
|
| Hybridization protocol |
10 ug fragmented IVT cRNA was hybridized overnight at 45 Cat 60 rpm to Arabidopsis ATH1 arrays
|
| Scan protocol |
Arrays were scanned using Affymetrix 3000 G7 scanner
|
| Description |
gene expression data from unpollinated pistils
|
| Data processing |
Quality control was performed using Expression Console, differential gene expression was detected using Partek Genomics Suite and RMA was used for normalization
|
| |
|
| Submission date |
Feb 19, 2013 |
| Last update date |
Jun 18, 2013 |
| Contact name |
Alexander Rivinus Leydon |
| E-mail(s) |
Alexander_leydon@brown.edu
|
| Phone |
401-863-6122
|
| Organization name |
Brown University
|
| Department |
MCB
|
| Lab |
Johnson Lab
|
| Street address |
60 Olive Street
|
| City |
Providence |
| State/province |
RI |
| ZIP/Postal code |
02912 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE44405 |
Three MYB Transcription Factors Control Pollen Tube Differentiation Required for Sperm Release |
|
