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Sample GSM108352 Query DataSets for GSM108352
Status Public on Dec 31, 2006
Title smoker 3
Sample type RNA
Source name human CD3 positive T cells
Organism Homo sapiens
Characteristics smoker
Treatment protocol T -lymphocytes were precipitated untouched from 4ml peripheral blood mononuclear cells, washed in PBS + 2mM EDTA, lysed in TriZol
Extracted molecule total RNA
Extraction protocol the RNA was worked up using PLG Tubes and the Qiagen Rneasy Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 microg total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG U133A2.0 GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Description Gene expression data from the smoking participant 3
Data processing The data were analyzed with GCOS 1.4 using Affymetrix default analysis settings and global scaling as normalization method.
Submission date May 10, 2006
Last update date Jun 22, 2006
Contact name Sandy Mosig
Phone +49-3641-934813
Fax +49-3641-933950
Organization name University Hospital Jena
Department Molecular Hemostaseology - Institute of Vascular Medicine
Street address Bachstrasse 18
City Jena
ZIP/Postal code 07743
Country Germany
Platform ID GPL571
Series (1)
GSE4806 Gene expression profiles of T-lymphocytes are sensitive to the influence of heavy smoking

Data table header descriptions
ID_REF Affymetrix annotation number
VALUE GCOS 1.4 calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 814.9 P 0.00039
AFFX-BioB-M_at 1078.7 P 0.000044
AFFX-BioB-3_at 705.7 P 0.00007
AFFX-BioC-5_at 2463.7 P 0.000044
AFFX-BioC-3_at 3068.4 P 0.000044
AFFX-BioDn-5_at 5417.5 P 0.000044
AFFX-BioDn-3_at 10895.3 P 0.000052
AFFX-CreX-5_at 26143.6 P 0.000052
AFFX-CreX-3_at 32276.2 P 0.000044
AFFX-DapX-5_at 684.8 P 0.000095
AFFX-DapX-M_at 1560.6 P 0.000509
AFFX-DapX-3_at 1895 P 0.000044
AFFX-LysX-5_at 89.1 P 0.004995
AFFX-LysX-M_at 218.9 P 0.01667
AFFX-LysX-3_at 286.4 P 0.000052
AFFX-PheX-5_at 142.5 P 0.000195
AFFX-PheX-M_at 228.3 P 0.002556
AFFX-PheX-3_at 223.2 P 0.000169
AFFX-ThrX-5_at 175.7 P 0.000509
AFFX-ThrX-M_at 333.2 P 0.00007

Total number of rows: 22277

Table truncated, full table size 591 Kbytes.

Supplementary data files not provided

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