GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM108349 Query DataSets for GSM108349
Status Public on Dec 31, 2006
Title control nonsmoker 2
Sample type RNA
Source name human CD3 positive T cells
Organism Homo sapiens
Characteristics nonsmoker
Treatment protocol T -lymphocytes were precipitated untouched from 4ml peripheral blood mononuclear cells, washed in PBS + 2mM EDTA, lysed in TriZol
Extracted molecule total RNA
Extraction protocol the RNA was worked up using PLG Tubes and the Qiagen Rneasy Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 microg total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG U133A2.0 GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Description Gene expression data from the non smoking participant 2
Data processing The data were analyzed with GCOS 1.4 using Affymetrix default analysis settings and global scaling as normalization method.
Submission date May 10, 2006
Last update date Jun 22, 2006
Contact name Sandy Mosig
Phone +49-3641-934813
Fax +49-3641-933950
Organization name University Hospital Jena
Department Molecular Hemostaseology - Institute of Vascular Medicine
Street address Bachstrasse 18
City Jena
ZIP/Postal code 07743
Country Germany
Platform ID GPL571
Series (1)
GSE4806 Gene expression profiles of T-lymphocytes are sensitive to the influence of heavy smoking

Data table header descriptions
ID_REF Affymetrix annotation number
VALUE GCOS 1.4 calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 677.6 P 0.00034
AFFX-BioB-M_at 867 P 0.000052
AFFX-BioB-3_at 473.2 P 0.000127
AFFX-BioC-5_at 2116.7 P 0.000044
AFFX-BioC-3_at 2753.7 P 0.000044
AFFX-BioDn-5_at 4199.5 P 0.000044
AFFX-BioDn-3_at 10883 P 0.000052
AFFX-CreX-5_at 23931.3 P 0.000052
AFFX-CreX-3_at 31647.8 P 0.000044
AFFX-DapX-5_at 845.7 P 0.000081
AFFX-DapX-M_at 1953.1 P 0.000662
AFFX-DapX-3_at 2180 P 0.00007
AFFX-LysX-5_at 133.4 P 0.000509
AFFX-LysX-M_at 210.6 P 0.039661
AFFX-LysX-3_at 402.1 P 0.00011
AFFX-PheX-5_at 235.3 P 0.000297
AFFX-PheX-M_at 259.9 P 0.000754
AFFX-PheX-3_at 283.6 P 0.000044
AFFX-ThrX-5_at 286.7 P 0.003212
AFFX-ThrX-M_at 382.6 P 0.000095

Total number of rows: 22277

Table truncated, full table size 592 Kbytes.

Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap