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Sample GSM1081132 Query DataSets for GSM1081132
Status Public on Feb 12, 2013
Title 2M_ControlvsAlcohol
Sample type RNA
 
Channel 1
Source name pooled RNA from six control mice
Organism Mus musculus
Characteristics tissue: liver
time-point: 2 month treatment
strain: 129/Sv
Sex: male
treatment: none
Treatment protocol Alcohol-treated mice were fed ad libitum a liquid diet containing 4% ethanol. Control animals were fed an isocaloric diet supplemented with maltose dextran
Growth protocol Mice were 6-8 weeks old when the treatment started
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol followed by RNeasy Midi kit
Label Cy3
Label protocol Invitrogen SuperScript indirect cDNA labeling system
 
Channel 2
Source name pooled RNA from six alcohol-treated mice
Organism Mus musculus
Characteristics tissue: liver
time-point: 2 month treatment
strain: 129/Sv
Sex: male
treatment: alcohol-treated
Treatment protocol Alcohol-treated mice were fed ad libitum a liquid diet containing 4% ethanol. Control animals were fed an isocaloric diet supplemented with maltose dextran
Growth protocol Mice were 6-8 weeks old when the treatment started
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol followed by RNeasy Midi kit
Label Cy5
Label protocol Invitrogen SuperScript indirect cDNA labeling system
 
 
Hybridization protocol follow Agilent's two-color expression array hybridization protocol
Scan protocol Images were quantified using Agilent Feature Extraction Software (version 9.1).
Data processing Feature Extraction version 9.1was used to filter, normalize, and calculate the signal intensities and Cy5/Cy3 ratios.
 
Submission date Feb 11, 2013
Last update date Feb 12, 2013
Contact name Henghong Li
E-mail(s) hl234@georgetown.edu
Organization name Georgetown University
Department Biochemistry
Lab Fornace
Street address 3970 Reservoir Rd., NW
City Washington
State/province DC
ZIP/Postal code 20057
Country USA
 
Platform ID GPL7202
Series (1)
GSE44237 Gene expression changes in liver tissues from alcohol treated mice

Data table header descriptions
ID_REF
VALUE Normalized log10 ratio (alcohol-treated/control)

Data table
ID_REF VALUE
(-)3xSLv1 0.00E+00
(+)E1A_r60_1 2.48E-01
(+)E1A_r60_3 1.20E-01
(+)E1A_r60_a104 1.19E-01
(+)E1A_r60_a107 7.08E-02
(+)E1A_r60_a135 6.04E-02
(+)E1A_r60_a20 -6.08E-02
(+)E1A_r60_a22 -2.38E-02
(+)E1A_r60_a97 -1.01E-02
(+)E1A_r60_n11 1.52E-01
(+)E1A_r60_n9 -5.10E-02
(+)eQC-39 9.25E-02
(+)eQC-40 -4.08E-02
(+)eQC-41 2.29E-02
(+)eQC-42 -1.70E-01
A_51_P100021 1.99E-01
A_51_P100034 -7.34E-02
A_51_P100052 -6.64E-02
A_51_P100063 -4.03E-01
A_51_P100084 1.06E-01

Total number of rows: 41267

Table truncated, full table size 903 Kbytes.




Supplementary file Size Download File type/resource
GSM1081132_251486823749_wt2Mpos_2_1_1.txt.gz 11.8 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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