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Sample GSM1071416 Query DataSets for GSM1071416
Status Public on Dec 31, 2013
Title Normal-4 [miRNA]
Sample type RNA
 
Source name non-neoplastic pancreas
Organism Homo sapiens
Characteristics tissue: non-neoplastic pancreas
Extracted molecule total RNA
Extraction protocol Total RNA was obtained from each independent tissue of patient and extracted using TRIzol Reagent (Invitrogen).
Label Cy3-pCp
Label protocol 100ng total RNA was dephosphorylated at the presence of calf intestine alkaline phsphatase, then a Cy3-pCp molecule was then ligated to the 3' end of the RNA molecule according to Agilent miRNA protocol version 1.0
 
Hybridization protocol Human microRNA Microarray Kit (Agilent Technologies) according to manufacture’s protocol. (http://www.chem.agilent.com/en-US/Pages/Homepage.aspx)
Scan protocol Scanned on an Agilent C scanner. Images were quantified using Agilent Feature Extraction Software ( v 10.10.1.1).
Description N-4
Data processing Raw data were extracted using the Agilent Feature Extraction Software (v 10.7.1.1) and then processed as published previously <Processing of Agilent microRNA array data, BMC RES NOTES,2010>
 
Submission date Jan 28, 2013
Last update date Dec 31, 2013
Contact name minhee park
Organization name student
Street address shinchondong
City seoul
ZIP/Postal code 120-752
Country South Korea
 
Platform ID GPL15159
Series (2)
GSE43796 microRNA expression of pancreatic tumors
GSE43797 Characterization of mRNA and microRNA expression profiles in solid-pseudopapillary neoplasm of pancreas, ductal adenocarcinoma and pancreatic neuroendocrine tumors

Data table header descriptions
ID_REF
VALUE The value was log2 transformed and quantile normalized

Data table
ID_REF VALUE
A_25_P00010019 6.139738286
A_25_P00010020 5.808928675
A_25_P00010021 5.611423329
A_25_P00010023 5.838842629
A_25_P00010041 5.678156985
A_25_P00010042 5.538967512
A_25_P00010043 5.562636481
A_25_P00010044 5.463181437
A_25_P00010047 9.52902618
A_25_P00010048 7.259097451
A_25_P00010053 12.75573548
A_25_P00010054 10.42091513
A_25_P00010062 7.260679383
A_25_P00010063 6.132900757
A_25_P00010070 14.36118369
A_25_P00010071 13.56619169
A_25_P00010072 12.99260717
A_25_P00010073 11.14185094
A_25_P00010078 9.917689301
A_25_P00010079 7.868540683

Total number of rows: 3523

Table truncated, full table size 92 Kbytes.




Supplementary file Size Download File type/resource
GSM1071416_N-4.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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