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Sample GSM1071409 Query DataSets for GSM1071409
Status Public on Dec 31, 2013
Title NET-3 [miRNA]
Sample type RNA
 
Source name neuroendocrine tumor
Organism Homo sapiens
Characteristics gender: female
age: 49
tissue: neuroendocrine tumor (NET)
Extracted molecule total RNA
Extraction protocol Total RNA was obtained from each independent tissue of patient and extracted using TRIzol Reagent (Invitrogen).
Label Cy3-pCp
Label protocol 100ng total RNA was dephosphorylated at the presence of calf intestine alkaline phsphatase, then a Cy3-pCp molecule was then ligated to the 3' end of the RNA molecule according to Agilent miRNA protocol version 1.0
 
Hybridization protocol Human microRNA Microarray Kit (Agilent Technologies) according to manufacture’s protocol. (http://www.chem.agilent.com/en-US/Pages/Homepage.aspx)
Scan protocol Scanned on an Agilent C scanner. Images were quantified using Agilent Feature Extraction Software ( v 10.10.1.1).
Description E-3
Data processing Raw data were extracted using the Agilent Feature Extraction Software (v 10.7.1.1) and then processed as published previously <Processing of Agilent microRNA array data, BMC RES NOTES,2010>
 
Submission date Jan 28, 2013
Last update date Dec 31, 2013
Contact name minhee park
Organization name student
Street address shinchondong
City seoul
ZIP/Postal code 120-752
Country South Korea
 
Platform ID GPL15159
Series (2)
GSE43796 microRNA expression of pancreatic tumors
GSE43797 Characterization of mRNA and microRNA expression profiles in solid-pseudopapillary neoplasm of pancreas, ductal adenocarcinoma and pancreatic neuroendocrine tumors

Data table header descriptions
ID_REF
VALUE The value was log2 transformed and quantile normalized

Data table
ID_REF VALUE
A_25_P00010019 6.404516178
A_25_P00010020 5.933898052
A_25_P00010021 5.751662588
A_25_P00010023 6.55322983
A_25_P00010041 5.683269406
A_25_P00010042 5.6026245
A_25_P00010043 5.488367206
A_25_P00010044 5.58469536
A_25_P00010047 10.2866117
A_25_P00010048 8.296079213
A_25_P00010053 14.88623604
A_25_P00010054 12.07941804
A_25_P00010062 8.819938658
A_25_P00010063 6.933874997
A_25_P00010070 12.77231894
A_25_P00010071 11.84637718
A_25_P00010072 11.19959983
A_25_P00010073 8.826682556
A_25_P00010078 6.591851495
A_25_P00010079 5.795657045

Total number of rows: 3523

Table truncated, full table size 92 Kbytes.




Supplementary file Size Download File type/resource
GSM1071409_E-3.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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