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Status
Public on Aug 11, 2006
Title
57_5dPA_MK02X022
Sample type
RNA
Source name
amputation blastema-proximal (1mm) tissue from Xenopus laevis hindlimbs
Organism
Xenopus laevis
Characteristics
Stage 57 Xenopus leavis hindlimbs, 5 day post-amputation
Extracted molecule
total RNA
Label
biotin
Description
Gene expression data from Hindlimbs at either st57 were amputated unilaterally or bilaterally at the mid-zeugopodia level. Five days (5dPA) after amputation tissues were collected 1mm proximal to the original level of amputation. Total RNA was isolated using RNaqueous micro kit (Ambion, inc.).
Data processing
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Submission date
Apr 28, 2006
Last update date
Aug 10, 2006
Contact name
Matthew Grow
Organization name
Indiana University
Department
Biochemistry and Molecular Biology
Lab
Center for Medical Genomics
Street address
1345 W 16th St
City
Indianapolis
State/province
IN
ZIP/Postal code
46202
Country
USA
Platform ID
GPL1318
Series (1)
GSE4738
Global analysis of gene expression in Xenopus hindlimbs during stage-dependent complete and incomplete regeneration
Data table header descriptions
ID_REF
VALUE
MAS5-calculated Signal intensity
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE
'detection p-value', p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-BioB-5_at
1558.3
P
0.000754
AFFX-BioB-M_at
2823.2
P
0.000258
AFFX-BioB-3_at
1612.2
P
0.000044
AFFX-BioC-5_at
6329.9
P
0.00007
AFFX-BioC-3_at
5569.1
P
0.000044
AFFX-BioDn-5_at
12155.4
P
0.000044
AFFX-BioDn-3_at
17571.8
P
0.00006
AFFX-CreX-5_at
54815.8
P
0.000044
AFFX-CreX-3_at
75749.9
P
0.000044
AFFX-DapX-5_at
183.8
P
0.030976
AFFX-DapX-M_at
1447
P
0.001102
AFFX-DapX-3_at
8959.5
P
0.00007
AFFX-LysX-5_at
185
P
0.018281
AFFX-LysX-M_at
1014.5
P
0.004017
AFFX-LysX-3_at
4099.1
P
0.000052
AFFX-PheX-5_at
157.1
M
0.050229
AFFX-PheX-M_at
188.9
A
0.300606
AFFX-PheX-3_at
2585.6
P
0.00006
AFFX-ThrX-5_at
14.9
A
0.990663
AFFX-ThrX-M_at
224.4
A
0.083592
Total number of rows: 15611 Table truncated, full table size 509 Kbytes .Supplementary data files not provided