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Sample GSM1068590 Query DataSets for GSM1068590
Status Public on Jan 20, 2015
Title C774
Sample type RNA
Source name Grupo1
Organism Homo sapiens
Characteristics tissue: skeletal muscle
gender: male
age: 2 years
sample type: mtDNA depletion
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from skeletal muscle biopsies with Qiagen (Qiagen, Hilden, Germany) RNeasy Fibrous Tissue mini kit following the manufacturer’s instructions
Label cy3
Label protocol RNA (500 ng) was labeled using Agilent's Low Input RNA Labeling Kit, which involves reverse transcribing the mRNA in the presence of T7-oligo-dT primer to produce cDNA and then in vitro transcribing with T7 RNA polymerase in the presence of Cy3-CTP
Hybridization protocol Labelled cRNAs were hybridised to the Agilent Human SurePrintGE, 8x60K according to manufacturer´s instructions
Scan protocol The arrays were washed, dried by centrifugation and scanned on an Agilent G2565BA microarray scanner at 100% PMT and 5 μm resolution
Description patient reference in corresponding publication: P3
Data processing Bioconductor limma package, using background correction (normexp) and quantiles normalization between arrays for final values
Submission date Jan 23, 2013
Last update date Jan 20, 2015
Contact name Susana Kalko
Organization name IDIBAPS
Department Bioinformatics Platform
Street address c/Rosello 153
City Barcelona
State/province BARCELONA
ZIP/Postal code 08036
Country Spain
Platform ID GPL13607
Series (1)
GSE43698 Muscle from patients with mitochondrial myopathies and congenital muscular dystrophies versus normal human muscle

Data table header descriptions
VALUE quantile normalized

Data table
4 8.629110014
5 6.766150063
6 6.530482308
7 11.83979469
8 8.640002508
9 6.074052488
10 6.520282224
11 6.262351825
12 9.257441341
13 9.375781083
14 7.826872723
15 7.246699478
16 6.005356284
17 6.945974699
18 6.038068034
19 5.990219101
20 9.558814604
21 8.795477636
22 6.197023183
23 6.158352227

Total number of rows: 62973

Table truncated, full table size 1089 Kbytes.

Supplementary file Size Download File type/resource
GSM1068590_13_MA_2100252800414336_Cy3_C7_74_2_1.txt.gz 12.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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