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Sample GSM1068589 Query DataSets for GSM1068589
Status Public on Jan 20, 2015
Title Tk2-02
Sample type RNA
Source name Grupo1
Organism Homo sapiens
Characteristics tissue: skeletal muscle
gender: male
age: 3 years
sample type: mtDNA depletion
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from skeletal muscle biopsies with Qiagen (Qiagen, Hilden, Germany) RNeasy Fibrous Tissue mini kit following the manufacturer’s instructions
Label cy3
Label protocol RNA (500 ng) was labeled using Agilent's Low Input RNA Labeling Kit, which involves reverse transcribing the mRNA in the presence of T7-oligo-dT primer to produce cDNA and then in vitro transcribing with T7 RNA polymerase in the presence of Cy3-CTP
Hybridization protocol Labelled cRNAs were hybridised to the Agilent Human SurePrintGE, 8x60K according to manufacturer´s instructions
Scan protocol The arrays were washed, dried by centrifugation and scanned on an Agilent G2565BA microarray scanner at 100% PMT and 5 μm resolution
Description patient reference in corresponding publication: P2
Data processing Bioconductor limma package, using background correction (normexp) and quantiles normalization between arrays for final values
Submission date Jan 23, 2013
Last update date Jan 20, 2015
Contact name Susana Kalko
Organization name IDIBAPS
Department Bioinformatics Platform
Street address c/Rosello 153
City Barcelona
State/province BARCELONA
ZIP/Postal code 08036
Country Spain
Platform ID GPL13607
Series (1)
GSE43698 Muscle from patients with mitochondrial myopathies and congenital muscular dystrophies versus normal human muscle

Data table header descriptions
VALUE quantile normalized

Data table
4 7.868927511
5 6.576596932
6 6.550743995
7 11.23719471
8 9.180477125
9 5.938050187
10 6.580290904
11 6.023634875
12 9.629278933
13 9.544936501
14 8.422269295
15 6.852687053
16 6.026486511
17 6.413223691
18 5.955676741
19 6.097414179
20 10.00155687
21 8.707664771
22 6.123653209
23 6.150073601

Total number of rows: 62973

Table truncated, full table size 1089 Kbytes.

Supplementary file Size Download File type/resource
GSM1068589_12_MA_1400252800414336_Cy3_Tk2_02_1_4.txt.gz 12.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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