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Sample GSM1068587 Query DataSets for GSM1068587
Status Public on Jan 20, 2015
Title B08-2833
Sample type RNA
 
Source name Grupo1
Organism Homo sapiens
Characteristics tissue: skeletal muscle
gender: female
age: 7 months
sample type: mtDNA depletion
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from skeletal muscle biopsies with Qiagen (Qiagen, Hilden, Germany) RNeasy Fibrous Tissue mini kit following the manufacturer’s instructions
Label cy3
Label protocol RNA (500 ng) was labeled using Agilent's Low Input RNA Labeling Kit, which involves reverse transcribing the mRNA in the presence of T7-oligo-dT primer to produce cDNA and then in vitro transcribing with T7 RNA polymerase in the presence of Cy3-CTP
 
Hybridization protocol Labelled cRNAs were hybridised to the Agilent Human SurePrintGE, 8x60K according to manufacturer´s instructions
Scan protocol The arrays were washed, dried by centrifugation and scanned on an Agilent G2565BA microarray scanner at 100% PMT and 5 μm resolution
Description patient reference in corresponding publication: P10
Data processing Bioconductor limma package, using background correction (normexp) and quantiles normalization between arrays for final values
 
Submission date Jan 23, 2013
Last update date Jan 20, 2015
Contact name Susana Kalko
E-mail(s) kalko.susana@gmail.com
Organization name IDIBAPS
Department Bioinformatics Platform
Street address c/Rosello 153
City Barcelona
State/province BARCELONA
ZIP/Postal code 08036
Country Spain
 
Platform ID GPL13607
Series (1)
GSE43698 Muscle from patients with mitochondrial myopathies and congenital muscular dystrophies versus normal human muscle

Data table header descriptions
ID_REF
VALUE quantile normalized

Data table
ID_REF VALUE
4 8.229151898
5 6.670894485
6 6.232149343
7 10.8308803
8 7.742629933
9 5.941006332
10 6.218142074
11 6.016321493
12 9.851839132
13 9.875802108
14 6.935907751
15 7.458401208
16 5.962416239
17 6.991692467
18 6.00050744
19 6.158935194
20 9.65045095
21 8.397781777
22 5.927201951
23 5.974912435

Total number of rows: 62973

Table truncated, full table size 1089 Kbytes.




Supplementary file Size Download File type/resource
GSM1068587_10_MA_1300252800414335_Cy3_B08-2833_1_3.txt.gz 12.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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