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Sample GSM1068585 Query DataSets for GSM1068585
Status Public on Jan 20, 2015
Title N10-54
Sample type RNA
Source name Control
Organism Homo sapiens
Characteristics tissue: skeletal muscle
gender: female
age: 1 year
sample type: normal muscle
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from skeletal muscle biopsies with Qiagen (Qiagen, Hilden, Germany) RNeasy Fibrous Tissue mini kit following the manufacturer’s instructions
Label cy3
Label protocol RNA (500 ng) was labeled using Agilent's Low Input RNA Labeling Kit, which involves reverse transcribing the mRNA in the presence of T7-oligo-dT primer to produce cDNA and then in vitro transcribing with T7 RNA polymerase in the presence of Cy3-CTP
Hybridization protocol Labelled cRNAs were hybridised to the Agilent Human SurePrintGE, 8x60K according to manufacturer´s instructions
Scan protocol The arrays were washed, dried by centrifugation and scanned on an Agilent G2565BA microarray scanner at 100% PMT and 5 μm resolution
Description patient reference in corresponding publication: C4
Data processing Bioconductor limma package, using background correction (normexp) and quantiles normalization between arrays for final values
Submission date Jan 23, 2013
Last update date Jan 20, 2015
Contact name Susana Kalko
Organization name IDIBAPS
Department Bioinformatics Platform
Street address c/Rosello 153
City Barcelona
State/province BARCELONA
ZIP/Postal code 08036
Country Spain
Platform ID GPL13607
Series (1)
GSE43698 Muscle from patients with mitochondrial myopathies and congenital muscular dystrophies versus normal human muscle

Data table header descriptions
VALUE quantile normalized

Data table
4 8.043856281
5 6.703014459
6 7.045944784
7 11.1752396
8 8.694932645
9 6.100956783
10 6.572624939
11 6.136625966
12 10.36829307
13 9.414833223
14 8.835042279
15 6.685996678
16 6.136209884
17 7.057331688
18 6.790825814
19 6.490908044
20 10.1320479
21 7.845058437
22 6.260253302
23 6.271567791

Total number of rows: 62973

Table truncated, full table size 1089 Kbytes.

Supplementary file Size Download File type/resource
GSM1068585_25_MA_2400252800414315_Cy3_N10-54_2_4.txt.gz 12.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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