|
| Status |
Public on Jan 20, 2015 |
| Title |
92B-1025 |
| Sample type |
RNA |
| |
|
| Source name |
Pat 2
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: skeletal muscle
gender: male
age: 4 years
sample type: Duchenne Muscular Dystrophy
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from skeletal muscle biopsies with Qiagen (Qiagen, Hilden, Germany) RNeasy Fibrous Tissue mini kit following the manufacturer’s instructions
|
| Label |
cy3
|
| Label protocol |
RNA (500 ng) was labeled using Agilent's Low Input RNA Labeling Kit, which involves reverse transcribing the mRNA in the presence of T7-oligo-dT primer to produce cDNA and then in vitro transcribing with T7 RNA polymerase in the presence of Cy3-CTP
|
| |
|
| Hybridization protocol |
Labelled cRNAs were hybridised to the Agilent Human SurePrintGE, 8x60K according to manufacturer´s instructions
|
| Scan protocol |
The arrays were washed, dried by centrifugation and scanned on an Agilent G2565BA microarray scanner at 100% PMT and 5 μm resolution
|
| Description |
patient reference in corresponding publication: DMD1
|
| Data processing |
Bioconductor limma package, using background correction (normexp) and quantiles normalization between arrays for final values
|
| |
|
| Submission date |
Jan 23, 2013 |
| Last update date |
Jan 20, 2015 |
| Contact name |
Susana Kalko |
| E-mail(s) |
kalko.susana@gmail.com
|
| Organization name |
IDIBAPS
|
| Department |
Bioinformatics Platform
|
| Street address |
c/Rosello 153
|
| City |
Barcelona |
| State/province |
BARCELONA |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL13607 |
| Series (1) |
| GSE43698 |
Muscle from patients with mitochondrial myopathies and congenital muscular dystrophies versus normal human muscle |
|
