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Sample GSM1068575 Query DataSets for GSM1068575
Status Public on Jan 20, 2015
Title 92B-1025
Sample type RNA
Source name Pat 2
Organism Homo sapiens
Characteristics tissue: skeletal muscle
gender: male
age: 4 years
sample type: Duchenne Muscular Dystrophy
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from skeletal muscle biopsies with Qiagen (Qiagen, Hilden, Germany) RNeasy Fibrous Tissue mini kit following the manufacturer’s instructions
Label cy3
Label protocol RNA (500 ng) was labeled using Agilent's Low Input RNA Labeling Kit, which involves reverse transcribing the mRNA in the presence of T7-oligo-dT primer to produce cDNA and then in vitro transcribing with T7 RNA polymerase in the presence of Cy3-CTP
Hybridization protocol Labelled cRNAs were hybridised to the Agilent Human SurePrintGE, 8x60K according to manufacturer´s instructions
Scan protocol The arrays were washed, dried by centrifugation and scanned on an Agilent G2565BA microarray scanner at 100% PMT and 5 μm resolution
Description patient reference in corresponding publication: DMD1
Data processing Bioconductor limma package, using background correction (normexp) and quantiles normalization between arrays for final values
Submission date Jan 23, 2013
Last update date Jan 20, 2015
Contact name Susana Kalko
Organization name IDIBAPS
Department Bioinformatics Platform
Street address c/Rosello 153
City Barcelona
State/province BARCELONA
ZIP/Postal code 08036
Country Spain
Platform ID GPL13607
Series (1)
GSE43698 Muscle from patients with mitochondrial myopathies and congenital muscular dystrophies versus normal human muscle

Data table header descriptions
VALUE quantile normalized

Data table
4 8.347214108
5 6.653020933
6 6.155685682
7 11.59464782
8 9.403578867
9 7.227745923
10 6.220068033
11 5.949728234
12 9.910327675
13 9.684733307
14 8.250289356
15 6.429457301
16 6.036959066
17 6.853985212
18 5.956634037
19 6.31487931
20 10.03424413
21 8.320692948
22 6.047784078
23 6.041348751

Total number of rows: 62973

Table truncated, full table size 1089 Kbytes.

Supplementary file Size Download File type/resource
GSM1068575_07_MA_1100252800414336_Cy3_92B-1025_1_1.txt.gz 12.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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