|
| Status |
Public on May 20, 2015 |
| Title |
RWPE1-PI3K rep1 |
| Sample type |
RNA |
| |
|
| Source name |
RWPE1 (Benign prostate epithelial cell line)
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: constitutive active PI3K (E545K)
cell line: RWPE1
|
| Treatment protocol |
There is no treatment prior to harvesting of cells
|
| Growth protocol |
RWPE1 and all its derivative sub-lines were grown in K-SFM with 50mg/ml BPE, 5ng/ml EGF (Invitrogen). All media were supplemented with 5000U/mL penicillin/streptomycin (Invitrogen). All cells were maintained at 37 °C with 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Retroviral infection to insert vector
|
| Data processing |
The data were normalised using quantile normalisation with Genome Studio
|
| |
|
| Submission date |
Jan 23, 2013 |
| Last update date |
May 20, 2015 |
| Contact name |
Zhen Ning Wee |
| E-mail(s) |
adrian.wzn@gmail.com
|
| Organization name |
Genome Institute of Singapore
|
| Lab |
Cancer therapeutics & stratified oncology
|
| Street address |
60 Biopolis
|
| City |
singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE43686 |
Myc and PI3-k induced gene expression in RWPE1 |
|
