|
Status |
Public on Apr 01, 2013 |
Title |
252800411248-5 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
A549 pmiR-67 24h
|
Organism |
Homo sapiens |
Characteristics |
cell line: A549 amount in (µg): 0.2 origin: Lung
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen
|
Label |
Cy3
|
Label protocol |
Agilent - Low RNA input QuickAmp
|
|
|
Channel 2 |
Source name |
A549 pmiR-210 24h
|
Organism |
Homo sapiens |
Characteristics |
cell line: A549 amount in (µg): 0.2 origin: Lung
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen
|
Label |
Cy5
|
Label protocol |
Agilent - Low RNA input QuickAmp
|
|
|
|
Hybridization protocol |
Agilent : 600.0 ng at 65 degree_C during 17 hours
|
Scan protocol |
Arrays were scanned using the Agilent SureScan High Resolution Scanner.
|
Description |
A549 pmiR-67 24h vs A549 pmiR-210 24h
|
Data processing |
LimmaGUI differential expression analysis with lowess intra-slide normalization and quantile inter-slide normalization.
|
|
|
Submission date |
Jan 18, 2013 |
Last update date |
Apr 01, 2013 |
Contact name |
Kevin Lebrigand |
Organization name |
IPMC/CNRS
|
Lab |
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
|
Street address |
660 route des lucioles
|
City |
Valbonne - Sophia-Antipolis |
ZIP/Postal code |
06560 |
Country |
France |
|
|
Platform ID |
GPL13607 |
Series (1) |
GSE43622 |
miR-210 promotes a hypoxic phenotype and increases radioresistance in human lung cancer cell lines. |
|