|
| Status |
Public on Apr 01, 2013 |
| Title |
252800411248-8 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
A549 pmiR-210 48h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
amount in (µg): 0.2
origin: Lung
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen
|
| Label |
Cy3
|
| Label protocol |
Agilent - Low RNA input QuickAmp
|
| |
|
| Channel 2 |
| Source name |
A549 pmiR-67 48h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
amount in (µg): 0.2
origin: Lung
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen
|
| Label |
Cy5
|
| Label protocol |
Agilent - Low RNA input QuickAmp
|
| |
|
| |
| Hybridization protocol |
Agilent : 600.0 ng at 65 degree_C during 17 hours
|
| Scan protocol |
Arrays were scanned using the Agilent SureScan High Resolution Scanner.
|
| Description |
A549 pmiR-210 48h vs A549 pmiR-67 48h
|
| Data processing |
LimmaGUI differential expression analysis with lowess intra-slide normalization and quantile inter-slide normalization.
|
| |
|
| Submission date |
Jan 18, 2013 |
| Last update date |
Apr 01, 2013 |
| Contact name |
Kevin Lebrigand |
| Organization name |
IPMC/CNRS
|
| Lab |
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
|
| Street address |
660 route des lucioles
|
| City |
Valbonne - Sophia-Antipolis |
| ZIP/Postal code |
06560 |
| Country |
France |
| |
|
| Platform ID |
GPL13607 |
| Series (1) |
| GSE43622 |
miR-210 promotes a hypoxic phenotype and increases radioresistance in human lung cancer cell lines. |
|
