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Sample GSM1058817 Query DataSets for GSM1058817
Status Public on Jan 01, 2013
Title LC24_rest
Sample type RNA
 
Source name skeletal muscle, resting
Organism Homo sapiens
Characteristics gender: female
insulin sensitivity - m value: 6.3
tissue: skeletal muscle
exercise status: before exercise
Treatment protocol volunteers had muscle biopsies at rest and 30 minutes after an exercise bout, and muscle biopsies were frozen in liquid nitrogen.
Extracted molecule total RNA
Extraction protocol Muscle biopsy specimens were homogenized in RNAStat solution using a Polytron homogenizer, and the total RNA was purified with RNeasy and DNase I treatment.
Label Cy3
Label protocol Total RNA was amplified and labeled using the Amino Allyl MessageAmp II aRNA amplification kit and quantified for Cy3 dye labeled aRNA concentration, RNA absorbance ratio, A260, Adye, and dye/probe values.
 
Hybridization protocol Cy3-labeled aRNA was fragmented using Agilent Gene Expression Hybridization kit and hybidized to 4x44K Whole Human Genome Microarray at 65C for 17 hours
Scan protocol After washing, slides were scanned with an Agilent DNA microarray scanner
Description Gene expression before exercise
US92200302_251485067006_S01_GE1_105_Jan09_1_1
Data processing Feature extraction software version 10.5.1.1 (Agilent) was used for analysis.
 
Submission date Dec 31, 2012
Last update date Jan 01, 2013
Contact name Dawn K Coletta
E-mail(s) dcoletta@deptofmed.arizona.edu
Phone 520-626-9316
Organization name UNIVERSITY OF ARIZONA
Department Medicine
Street address 1501 N. Campbell Ave
City Tucson
State/province AZ
ZIP/Postal code 85724
Country USA
 
Platform ID GPL4133
Series (1)
GSE43219 mRNA changes induced by acute exercise

Data table header descriptions
ID_REF
VALUE quantile normalized log2(signal intensities)

Data table
ID_REF VALUE
1 1.66E+01
2 5.95E+00
3 5.96E+00
4 6.20E+00
5 5.99E+00
6 5.98E+00
7 5.98E+00
8 6.02E+00
9 5.94E+00
10 5.94E+00
11 5.92E+00
12 7.22E+00
13 6.64E+00
14 7.92E+00
15 6.70E+00
16 1.01E+01
17 7.10E+00
18 9.39E+00
19 1.43E+01
20 6.78E+00

Total number of rows: 45015

Table truncated, full table size 648 Kbytes.




Supplementary file Size Download File type/resource
GSM1058817_US92200302_251485067006_S01_GE1_105_Jan09_1_1.txt.gz 7.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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