NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1054199 Query DataSets for GSM1054199
Status Public on Dec 19, 2012
Title LPSX3 cell culture
Sample type RNA
 
Channel 1
Source name Mixed reference pool of fatty tumors
Organism Homo sapiens
Characteristics reference: total RNA from human 8 benign fatty tumors (7 lipomas, 1 hibernoma), 33 well differentitated liposarcoma, 15 de-differentiated liposarcoma, 2 pleomorphic liposarcoma, and 11 myxoid liposarcomas
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using QIAzol RNA Kit following manufacturer's instructions
Label Cy3
Label protocol Using Agilent Quick Amp Labeling Kit (Agilent Technologies) and protocol
 
Channel 2
Source name LPSX3 cell culture
Organism Homo sapiens
Characteristics tissue type: cell culture
cell type: liposarcoma
tumor histology: de-differentiated
host organism: NA
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using QIAzol RNA Kit following manufacturer's instructions
Label Cy5
Label protocol Using Agilent Quick Amp Labeling Kit (Agilent Technologies) and protocol
 
 
Hybridization protocol Following Agilent Human 44K microarray processing protocol
Scan protocol Scanned on an Agilent Scanner
Description cultured cells derived from xenograft tumor of LPSX3
Data processing Agilent Feature Extraction software v.7.5 was used to calculate gene expression values. For multiple spots representing each unique probe, the average of their intensity ratios (log10 of Cy5/Cy3) was used to represent the probe.
 
Submission date Dec 18, 2012
Last update date Dec 19, 2012
Contact name Linh My Tran
E-mail(s) linhtran@ucla.edu
Organization name University of Los Angeles
Department Medicine - Pulmonary & Critical Care
Lab Dubinett Lab
Street address Box 951690, 37-131 CHS
City Los Angeles
State/province California
ZIP/Postal code 90095-1690
Country USA
 
Platform ID GPL6480
Series (1)
GSE42975 Novel dedifferentiated liposarcoma xenograft models reveal PTEN down regulation as a malignant signature and response to PI3K pathway inhibition

Data table header descriptions
ID_REF
VALUE log10 ratio (Cy5/Cy3) representing sample/reference

Data table
ID_REF VALUE
A_23_P100001 -0.243690622
A_23_P100011 0.233553703
A_23_P100022 -0.740040221
A_23_P100056 -0.152039802
A_23_P100074 0.410493877
A_23_P100092 -0.008414217
A_23_P100103 -0.03139923
A_23_P100111 0.069057078
A_23_P100127 0.082533903
A_23_P100133 -0.029546472
A_23_P100141 0.238057187
A_23_P100156 0.123480956
A_23_P100177 0.034157005
A_23_P100189 0.012942566
A_23_P100196 0.181008239
A_23_P100203 0.193530671
A_23_P100220 0.076630307
A_23_P100240 -0.039331512
A_23_P10025 -0.349549252
A_23_P100263 -0.053939447

Total number of rows: 41000

Table truncated, full table size 1001 Kbytes.




Supplementary file Size Download File type/resource
GSM1054199_B02_251485061342_S01_GE2-v5_95_Feb07_2_Off_1_2.txt.gz 14.2 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap