|
| Status |
Public on Dec 19, 2012 |
| Title |
LPSX3 Xenograft passage 6 mechanic dissociation |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Mixed reference pool of fatty tumors
|
| Organism |
Homo sapiens |
| Characteristics |
reference: total RNA from human 8 benign fatty tumors (7 lipomas, 1 hibernoma), 33 well differentitated liposarcoma, 15 de-differentiated liposarcoma, 2 pleomorphic liposarcoma, and 11 myxoid liposarcomas
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using QIAzol RNA Kit following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Using Agilent Quick Amp Labeling Kit (Agilent Technologies) and protocol
|
| |
|
| Channel 2 |
| Source name |
LPSX3 Xenograft passage 6 mechanical dissociation
|
| Organism |
Homo sapiens |
| Characteristics |
tissue type: xenograft
cell type: liposarcoma
tumor histology: de-differentiated
host organism: Mus musculus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using QIAzol RNA Kit following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
Using Agilent Quick Amp Labeling Kit (Agilent Technologies) and protocol
|
| |
|
| |
| Hybridization protocol |
Following Agilent Human 44K microarray processing protocol
|
| Scan protocol |
Scanned on an Agilent Scanner
|
| Description |
Xenograft tumor of LPSX3 at passage 6; mechanical dissociated cells were used in the subcutaneous injection
|
| Data processing |
Agilent Feature Extraction software v.7.5 was used to calculate gene expression values. For multiple spots representing each unique probe, the average of their intensity ratios (log10 of Cy5/Cy3) was used to represent the probe.
|
| |
|
| Submission date |
Dec 18, 2012 |
| Last update date |
Dec 19, 2012 |
| Contact name |
Linh My Tran |
| E-mail(s) |
linhtran@ucla.edu
|
| Organization name |
University of Los Angeles
|
| Department |
Medicine - Pulmonary & Critical Care
|
| Lab |
Dubinett Lab
|
| Street address |
Box 951690, 37-131 CHS
|
| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90095-1690 |
| Country |
USA |
| |
|
| Platform ID |
GPL6480 |
| Series (1) |
| GSE42975 |
Novel dedifferentiated liposarcoma xenograft models reveal PTEN down regulation as a malignant signature and response to PI3K pathway inhibition |
|
