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Sample GSM1054190 Query DataSets for GSM1054190
Status Public on Dec 19, 2012
Title LPSX2 cell culture
Sample type RNA
 
Channel 1
Source name Mixed reference pool of fatty tumors
Organism Homo sapiens
Characteristics reference: total RNA from human 8 benign fatty tumors (7 lipomas, 1 hibernoma), 33 well differentitated liposarcoma, 15 de-differentiated liposarcoma, 2 pleomorphic liposarcoma, and 11 myxoid liposarcomas
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using QIAzol RNA Kit following manufacturer's instructions
Label Cy3
Label protocol Using Agilent Quick Amp Labeling Kit (Agilent Technologies) and protocol
 
Channel 2
Source name LPSX2 cell culture
Organism Homo sapiens
Characteristics tissue type: cell culture
cell type: liposarcoma
tumor histology: de-differentiated
host organism: NA
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using QIAzol RNA Kit following manufacturer's instructions
Label Cy5
Label protocol Using Agilent Quick Amp Labeling Kit (Agilent Technologies) and protocol
 
 
Hybridization protocol Following Agilent Human 44K microarray processing protocol
Scan protocol Scanned on an Agilent Scanner
Description cultured cells derived from xenograft tumor of LPSX2
Data processing Agilent Feature Extraction software v.7.5 was used to calculate gene expression values. For multiple spots representing each unique probe, the average of their intensity ratios (log10 of Cy5/Cy3) was used to represent the probe.
 
Submission date Dec 18, 2012
Last update date Dec 19, 2012
Contact name Linh My Tran
E-mail(s) linhtran@ucla.edu
Organization name University of Los Angeles
Department Medicine - Pulmonary & Critical Care
Lab Dubinett Lab
Street address Box 951690, 37-131 CHS
City Los Angeles
State/province California
ZIP/Postal code 90095-1690
Country USA
 
Platform ID GPL6480
Series (1)
GSE42975 Novel dedifferentiated liposarcoma xenograft models reveal PTEN down regulation as a malignant signature and response to PI3K pathway inhibition

Data table header descriptions
ID_REF
VALUE log10 ratio (Cy5/Cy3) representing sample/reference

Data table
ID_REF VALUE
A_23_P100001 -0.370207859
A_23_P100011 0.192636942
A_23_P100022 -0.654464218
A_23_P100056 -0.224020308
A_23_P100074 -0.128859047
A_23_P100092 0.189175921
A_23_P100103 0.100459782
A_23_P100111 -0.347832706
A_23_P100127 0.489638145
A_23_P100133 -0.063300618
A_23_P100141 0.138836051
A_23_P100156 0.119504692
A_23_P100177 0.1349561
A_23_P100189 -0.293685145
A_23_P100196 0.137879352
A_23_P100203 0.505283713
A_23_P100220 0.224522262
A_23_P100240 -0.020376252
A_23_P10025 -0.395047652
A_23_P100263 -0.182940036

Total number of rows: 41000

Table truncated, full table size 1003 Kbytes.




Supplementary file Size Download File type/resource
GSM1054190_B02_251485061342_S01_GE2-v5_95_Feb07_2_Off_1_1.txt.gz 14.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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