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Sample GSM1054185 Query DataSets for GSM1054185
Status Public on Dec 19, 2012
Title LPSX1 Xenograft passage 15
Sample type RNA
 
Channel 1
Source name Mixed reference pool of fatty tumors
Organism Homo sapiens
Characteristics reference: total RNA from human 8 benign fatty tumors (7 lipomas, 1 hibernoma), 33 well differentitated liposarcoma, 15 de-differentiated liposarcoma, 2 pleomorphic liposarcoma, and 11 myxoid liposarcomas
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using QIAzol RNA Kit following manufacturer's instructions
Label Cy3
Label protocol Using Agilent Quick Amp Labeling Kit (Agilent Technologies) and protocol
 
Channel 2
Source name LPSX1 Xenograft passage 15
Organism Homo sapiens
Characteristics tissue type: xenograft
cell type: liposarcoma
tumor histology: de-differentiated
host organism: Mus musculus
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using QIAzol RNA Kit following manufacturer's instructions
Label Cy5
Label protocol Using Agilent Quick Amp Labeling Kit (Agilent Technologies) and protocol
 
 
Hybridization protocol Following Agilent Human 44K microarray processing protocol
Scan protocol Scanned on an Agilent Scanner
Description Xenograft tumor of LPSX1 at passage 15
Data processing Agilent Feature Extraction software v.7.5 was used to calculate gene expression values. For multiple spots representing each unique probe, the average of their intensity ratios (log10 of Cy5/Cy3) was used to represent the probe.
 
Submission date Dec 18, 2012
Last update date Dec 19, 2012
Contact name Linh My Tran
E-mail(s) linhtran@ucla.edu
Organization name University of Los Angeles
Department Medicine - Pulmonary & Critical Care
Lab Dubinett Lab
Street address Box 951690, 37-131 CHS
City Los Angeles
State/province California
ZIP/Postal code 90095-1690
Country USA
 
Platform ID GPL6480
Series (1)
GSE42975 Novel dedifferentiated liposarcoma xenograft models reveal PTEN down regulation as a malignant signature and response to PI3K pathway inhibition

Data table header descriptions
ID_REF
VALUE log10 ratio (Cy5/Cy3) representing sample/reference

Data table
ID_REF VALUE
A_23_P100001 -0.199794449
A_23_P100011 0.241759282
A_23_P100022 -0.856622007
A_23_P100056 0.229778909
A_23_P100074 0.475107157
A_23_P100092 0.083977243
A_23_P100103 -0.000359271
A_23_P100111 0.166542705
A_23_P100127 0.514451581
A_23_P100133 -0.051966529
A_23_P100141 0.020504801
A_23_P100156 0.137587707
A_23_P100177 -0.087757577
A_23_P100189 -0.022395995
A_23_P100196 0.426617679
A_23_P100203 0.390535038
A_23_P100220 0.068123641
A_23_P100240 -0.023249289
A_23_P10025 -0.495212051
A_23_P100263 -0.277700926

Total number of rows: 41000

Table truncated, full table size 1001 Kbytes.




Supplementary file Size Download File type/resource
GSM1054185_B02_251485061515_S01_GE2-v5_95_Feb07_2_Off_1_1.txt.gz 14.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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