|
| Status |
Public on May 22, 2014 |
| Title |
Donor1_siEBF1_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Human adipocytes transfected with siRNA targeting EBF1 for 48hours
|
| Organism |
Homo sapiens |
| Characteristics |
transfection: siRNA targeting EBF1
cell type: fully differentiated adipocytes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy Lipid Tissue Mini Kit (Qiagen, Hilden, Germany) according to the manufacturers’ instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNA were amplified and biotin labeled using the Whole Transcript (WT) Sense Target Labeling Protocol
|
| |
|
| Hybridization protocol |
Biotinylated RNAs were hybridized to Gene 1.1 ST Arrays, and then washed, stained and scanned slides using standardized protocols (Affymetrix Inc., Santa Clara, CA).
|
| Scan protocol |
Standardized Affymetrix protocols (Affymetrix Inc., Santa Clara, CA)
|
| Description |
Gene expression data from fully differentiated preadipocyte after transfected with siRNA tageting EBF1 for 48 hours
|
| Data processing |
Data were analyzed with packages available from Bioconductor (www.bioconductor.org). Normalization and calculation of gene expression was performed with the Robust Multichip Average expression measure using oligo package. Prior to further analysis, a non-specific filter was applied to include genes with expression signal >30 in at least 20% of all samples. Limma package was used to identify the differentially expressed genes
|
| |
|
| Submission date |
Dec 03, 2012 |
| Last update date |
May 22, 2014 |
| Contact name |
hui Gao |
| E-mail(s) |
hui.gao@ki.se
|
| Organization name |
Karolinska Institutet
|
| Department |
Department of Biosciences and Nutrition
|
| Street address |
Novum
|
| City |
Huddinge |
| ZIP/Postal code |
14183 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL11532 |
| Series (2) |
| GSE42680 |
Early B-cell Factor 1 Regulates Adipocyte Morphology and Lipolysis in White Adipose Tissue [expression profiling] |
| GSE54890 |
Early B-cell Factor 1 Regulates Adipocyte Morphology and Lipolysis in White Adipose Tissue |
|
