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Status |
Public on Sep 01, 2006 |
Title |
HUVEC/NmenMC58_frpC/frpA_4h_rep3_1117 |
Sample type |
RNA |
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Source name |
Human Umbilical Vein Endothelial Cells (HUVEC)
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Organism |
Homo sapiens |
Characteristics |
Tissue: endothelial cells
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Biomaterial provider |
PromoCell Cat. no. C-12200-human umbilical vein endothelial cells; INSERM U570, Faculté de Médecine René Descartes, Paris, France, prof. Xavier Nassif - Neisseria meningitidis MC58
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Treatment protocol |
Monolayers of HUVECs in 9 cm Petri dishes infected with Neisseria meningitidis MC58 delta frpC/frpA were washed and collected into Trizol by cell-scraper
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Growth protocol |
Confluent monolayers of HUVECs in 9 cm Petri dishes were infected with Neisseria meningitidis MC58 delta frpC/frpA (MOI=10) and cultivated in RPMI medium, 5% CO2, humidified atmosphere, 37°C, 4 hours
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Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was purified on RNeasy minicolumns (Qiagen) and by NaOAc/ethanol precipitation.
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Label |
biotin
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Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
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Hybridization protocol |
Following fragmentation, cRNA was hybridized for 16 hr at 45C on Affymetrix Human Genome HG-U133A GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station.
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Scan protocol |
GeneChips were scanned using the Agilent GeneArray scanner.
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Description |
Gene expression data from HUVEC infected with Neisseria meningitidis MC58 delta frpC/frpA, 4 hours infection
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Data processing |
The data were analyzed with RMA and MAS 5.0 algorithms using Biocondutor 1.7 and default analysis settings.
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Submission date |
Apr 10, 2006 |
Last update date |
Apr 10, 2006 |
Contact name |
Marek Basler |
E-mail(s) |
basler@biomed.cas.cz
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Phone |
+420 2 4106 2762
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URL |
http://l125lsx.mbu.cas.cz/Lab125/index.html
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Organization name |
Institute of Microbiology, Czech Academy of Sciences
|
Department |
Cell and Molecular Microbiology Division
|
Lab |
Laboratory of Molecular Biology of Bacterial Pathogens
|
Street address |
Videnska 1083
|
City |
Prague 4 |
ZIP/Postal code |
CZ-142 20 |
Country |
Czech Republic |
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|
Platform ID |
GPL96 |
Series (1) |
GSE4646 |
Signaling of Neisseria meningitidis MC58 mutants to primary human umbilical vein endothelial cells (HUVEC) |
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