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Sample GSM104494 Query DataSets for GSM104494
Status Public on Sep 01, 2006
Title HUVEC/NmenMC58_frpC/frpA_4h_rep2_1112
Sample type RNA
Source name Human Umbilical Vein Endothelial Cells (HUVEC)
Organism Homo sapiens
Characteristics Tissue: endothelial cells
Biomaterial provider PromoCell Cat. no. C-12200-human umbilical vein endothelial cells; INSERM U570, Faculté de Médecine René Descartes, Paris, France, prof. Xavier Nassif - Neisseria meningitidis MC58
Treatment protocol Monolayers of HUVECs in 9 cm Petri dishes infected with Neisseria meningitidis MC58 delta frpC/frpA were washed and collected into Trizol by cell-scraper
Growth protocol Confluent monolayers of HUVECs in 9 cm Petri dishes were infected with Neisseria meningitidis MC58 delta frpC/frpA (MOI=10) and cultivated in RPMI medium, 5% CO2, humidified atmosphere, 37°C, 4 hours
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was purified on RNeasy minicolumns (Qiagen) and by NaOAc/ethanol precipitation.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol Following fragmentation, cRNA was hybridized for 16 hr at 45C on Affymetrix Human Genome HG-U133A GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station.
Scan protocol GeneChips were scanned using the Agilent GeneArray scanner.
Description Gene expression data from HUVEC infected with Neisseria meningitidis MC58 delta frpC/frpA, 4 hours infection
Data processing The data were analyzed with RMA and MAS 5.0 algorithms using Biocondutor 1.7 and default analysis settings.
Submission date Apr 10, 2006
Last update date Apr 10, 2006
Contact name Marek Basler
Phone +420 2 4106 2762
Organization name Institute of Microbiology, Czech Academy of Sciences
Department Cell and Molecular Microbiology Division
Lab Laboratory of Molecular Biology of Bacterial Pathogens
Street address Videnska 1083
City Prague 4
ZIP/Postal code CZ-142 20
Country Czech Republic
Platform ID GPL96
Series (1)
GSE4646 Signaling of Neisseria meningitidis MC58 mutants to primary human umbilical vein endothelial cells (HUVEC)

Data table header descriptions
VALUE RMA-calculated Signal intensity
ABS_CALL indicates if the transcript was present (P), absent (A), marginal (M), based on MAS 5.0
DETECTION P-VALUE the significance level of the detection call, based on MAS 5.0
LOG2_VALUE Log2 transformed RMA-calculated Signal intensity, used for identification of significantly regulated genes

Data table
1007_s_at 346.5667053 P 0.004862793 8.436989251
1053_at 94.75106244 P 0.002617014 6.566070215
117_at 101.0850021 A 0.107301013 6.659425152
121_at 700.9464669 P 0.017000453 9.453160456
1255_g_at 24.3009932 A 0.107301013 4.602943374
1294_at 131.0835632 P 0.027859596 7.034342985
1316_at 60.66281378 P 0.035162814 5.922740512
1320_at 80.58825623 A 0.073830173 6.332497711
1405_i_at 28.29305771 A 0.9066256 4.822376196
1431_at 21.69125277 A 0.107301013 4.439041472
1438_at 105.1152834 A 0.541184238 6.715828637
1487_at 306.6499558 P 0.027859596 8.260448933
1494_f_at 158.3381778 M 0.048994635 7.306865344
1598_g_at 1311.987594 P 0.001141166 10.35753836
160020_at 783.316296 P 0.003066739 9.613451162
1729_at 273.931935 P 0.004180367 8.097673655
177_at 75.54472263 P 0.014936519 6.239259071
1773_at 80.88866508 A 0.218983229 6.337865647
179_at 905.7682041 A 0.107301013 9.822998086
1861_at 177.8723713 P 0.002617014 7.474698626

Total number of rows: 22283

Table truncated, full table size 1056 Kbytes.

Supplementary file Size Download File type/resource
GSM104494.CEL.gz 3.4 Mb (ftp)(http) CEL

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