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Sample GSM1038482 Query DataSets for GSM1038482
Status Public on Apr 04, 2013
Title 5 = B7_siGL3_96h
Sample type RNA
Source name hESCs_siGL3_96h
Organism Homo sapiens
Characteristics cell type: primary cultured human endometrial stromal cells (hESCs)
transfected with: control (GL3 luciferase) for 24 h
treated with: [E2+P4+cAMP] hormone cocktail for 96hr
Treatment protocol Independent hESC lines were established from individual biopsies of proliferative stage endometria, each cell line being from a different woman. These cultures were transfected with control (GL3 luciferase) or REA-specific siRNA for 24 h. siRNAs were then removed and cells were then treated for 24 h or 96 h in the presence of the hormone cocktail containing 10 nM E2, 1 µM progesterone and 0.5 µM 8Br-cAMP (E+P+cAMP). Cells were harvested and total RNA was prepared with TRIzol reagent. RNA integrity was verified using an Agilent 2100 Bioanalyser.
Growth protocol Human endometrial stromal cells were maintained in DMEM/F-12 medium containing 5% charcoal stripped fetal bovine serum
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G Plus.
Description 4417_E
Data processing The data were analyzed with GCOS2.1 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Submission date Nov 19, 2012
Last update date Apr 04, 2013
Contact name Yuechao Zhao
Organization name University of Illinois
Street address 407 S. Goodwin Ave
City Urbana
State/province IL
ZIP/Postal code 61801
Country USA
Platform ID GPL571
Series (1)
GSE42376 The coregulator, repressor of Estrogen Receptor Activity (REA), is a crucial regulator of the timing and magnitude of uterine decidualization.

Data table header descriptions
VALUE GCOS2.1 signal intensity

Data table
1007_s_at 878.25
1053_at 92.82527
117_at 17.90371
121_at 81.01653
1255_g_at 15.62938
1294_at 65.58772
1316_at 82.62472
1320_at 30.84892
1405_i_at 2.863027
1431_at 18.01397
1438_at 25.13434
1487_at 128.1182
1494_f_at 25.4914
1598_g_at 464.8017
160020_at 991.5658
1729_at 195.439
1773_at 45.59294
177_at 161.9591
179_at 82.46383
1861_at 320.9735

Total number of rows: 22277

Table truncated, full table size 432 Kbytes.

Supplementary file Size Download File type/resource
GSM1038482_4417_E_5_HG-U133A_2_.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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