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Sample GSM1038479 Query DataSets for GSM1038479
Status Public on Apr 04, 2013
Title 8 = N16_KO_96h
Sample type RNA
Source name hESCs_siREA_96h
Organism Homo sapiens
Characteristics cell type: primary cultured human endometrial stromal cells (hESCs)
transfected with: REA-specific siRNA for 24 h
treated with: [E2+P4+cAMP] hormone cocktail for 96hr
Treatment protocol Independent hESC lines were established from individual biopsies of proliferative stage endometria, each cell line being from a different woman. These cultures were transfected with control (GL3 luciferase) or REA-specific siRNA for 24 h. siRNAs were then removed and cells were then treated for 24 h or 96 h in the presence of the hormone cocktail containing 10 nM E2, 1 µM progesterone and 0.5 µM 8Br-cAMP (E+P+cAMP). Cells were harvested and total RNA was prepared with TRIzol reagent. RNA integrity was verified using an Agilent 2100 Bioanalyser.
Growth protocol Human endometrial stromal cells were maintained in DMEM/F-12 medium containing 5% charcoal stripped fetal bovine serum
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G Plus.
Description 4414_E
Data processing The data were analyzed with GCOS2.1 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Submission date Nov 19, 2012
Last update date Apr 04, 2013
Contact name Yuechao Zhao
Organization name University of Illinois
Street address 407 S. Goodwin Ave
City Urbana
State/province IL
ZIP/Postal code 61801
Country USA
Platform ID GPL571
Series (1)
GSE42376 The coregulator, repressor of Estrogen Receptor Activity (REA), is a crucial regulator of the timing and magnitude of uterine decidualization.

Data table header descriptions
VALUE GCOS2.1 signal intensity

Data table
1007_s_at 544.0881
1053_at 88.11992
117_at 17.67195
121_at 84.21072
1255_g_at 15.65965
1294_at 45.90022
1316_at 116.9535
1320_at 22.0827
1405_i_at 0.8326944
1431_at 13.88477
1438_at 22.53407
1487_at 84.82552
1494_f_at 16.01331
1598_g_at 293.2441
160020_at 921.4771
1729_at 212.6621
1773_at 34.45829
177_at 228.3595
179_at 64.76822
1861_at 265.4473

Total number of rows: 22277

Table truncated, full table size 432 Kbytes.

Supplementary file Size Download File type/resource
GSM1038479_4414_E_8_HG-U133A_2_.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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