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Sample GSM1038475 Query DataSets for GSM1038475
Status Public on Apr 04, 2013
Title 1 = B7_siGL3_24h
Sample type RNA
 
Source name hESCs_siGL3_24h
Organism Homo sapiens
Characteristics cell type: primary cultured human endometrial stromal cells (hESCs)
transfected with: control (GL3 luciferase) for 24 h
treated with: [E2+P4+cAMP] hormone cocktail for 24hr
Treatment protocol Independent hESC lines were established from individual biopsies of proliferative stage endometria, each cell line being from a different woman. These cultures were transfected with control (GL3 luciferase) or REA-specific siRNA for 24 h. siRNAs were then removed and cells were then treated for 24 h or 96 h in the presence of the hormone cocktail containing 10 nM E2, 1 µM progesterone and 0.5 µM 8Br-cAMP (E+P+cAMP). Cells were harvested and total RNA was prepared with TRIzol reagent. RNA integrity was verified using an Agilent 2100 Bioanalyser.
Growth protocol Human endometrial stromal cells were maintained in DMEM/F-12 medium containing 5% charcoal stripped fetal bovine serum
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G Plus.
Description 4410_E
Data processing The data were analyzed with GCOS2.1 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Nov 19, 2012
Last update date Apr 04, 2013
Contact name Yuechao Zhao
Organization name University of Illinois
Street address 407 S. Goodwin Ave
City Urbana
State/province IL
ZIP/Postal code 61801
Country USA
 
Platform ID GPL571
Series (1)
GSE42376 The coregulator, repressor of Estrogen Receptor Activity (REA), is a crucial regulator of the timing and magnitude of uterine decidualization.

Data table header descriptions
ID_REF
VALUE GCOS2.1 signal intensity

Data table
ID_REF VALUE
1007_s_at 383.3826
1053_at 72.20268
117_at 15.06718
121_at 92.92191
1255_g_at 22.72061
1294_at 121.0762
1316_at 109.2436
1320_at 34.29704
1405_i_at 0.004252283
1431_at 20.40563
1438_at 27.21292
1487_at 121.498
1494_f_at 18.48727
1598_g_at 1076.324
160020_at 824.3342
1729_at 262.6638
1773_at 32.81469
177_at 141.6757
179_at 80.27613
1861_at 356.3297

Total number of rows: 22277

Table truncated, full table size 432 Kbytes.




Supplementary file Size Download File type/resource
GSM1038475_4410_E_1_HG-U133A_2_.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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