|
| Status |
Public on May 08, 2006 |
| Title |
srb4 ts mutant ORF change-1 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
CHIP IP Med7-1 ORF in srb4 ts mutant
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
IP sample Med7-1 ORF in Srb4 ts mutant
|
| Extracted molecule |
genomic DNA |
| Label |
cy3
|
| |
|
| Channel 2 |
| Source name |
CHIP IP Med7-1 ORF in WT
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
IP sample Med7-1 ORF in WT
|
| Extracted molecule |
genomic DNA |
| Label |
cy5
|
| |
|
| |
| Description |
IgG beads were used for ChIP of chromatin extracts from Med7-TAP srb4 ts mutant and WT cells. Cell cultures were then grown to mid logarithmic growth phase (5 x106 cells/ml) in rich medium, then turn to 37 degree 90minutes. fixing cells with formaladehyde and ChIP. The resulting ChIP DNA was labeled with cy3 and Cy5 respectively. hybridized to the IGR+ORF
|
| Data processing |
cy3/cy5
|
| |
|
| Submission date |
Apr 04, 2006 |
| Last update date |
May 08, 2006 |
| Contact name |
Claes M Gustafsson |
| E-mail(s) |
claes.gustafsson@medkem.gu.se
|
| Organization name |
Gothenburg University
|
| Department |
Department of Medical biochemistry and cell biology
|
| Street address |
Medcinaregatan 9A
|
| City |
Gothenburg |
| ZIP/Postal code |
405 30 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL2789 |
| Series (1) |
| GSE3213 |
Genome-wide occupancy profile of mediator and the srb8-11 module reveals interactions with coding regions. |
|
