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Sample GSM1028106 Query DataSets for GSM1028106
Status Public on Feb 01, 2013
Title Reh cells_bortezomib_12h
Sample type RNA
Source name Reh cells, treatment with bortezomib, 12h
Organism Homo sapiens
Characteristics cell line: Reh
tissue: leukoblasts
Treatment protocol Reh cells were seeded at a denstity of 1x10E06 cells/ml in 12 well plates with corresponding drugs added from 100x stock solutions to achieve final concentrations.
Growth protocol RPMI 1640 Medium, 10%FCS, 1%Pen/Strep, 37°C, 5%CO2
Extracted molecule total RNA
Extraction protocol mRNA was extracted by using the RNeasy Kit (Quiagen).
Label cy3
Label protocol Labeling was performed according to the protocol using the Agilent Low Input Quick Amp Labeling Kit (Agilent)
Hybridization protocol Hybridization was performed according to the Agilent 60-mer oligo microarray processing protocol using the Agilent Gene Expression Hybridization Kit (Agilent)
Scan protocol Fluorescence signals of the hybridized Agilent Microarrays were detected using Agilent’s Microarray Scanner System (Agilent).
Data processing The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent). Further data analysis was performed using the Partek® Genomics Suits software, version 6.5 beta © 2009 (Partek Inc., USA).
Submission date Oct 31, 2012
Last update date Feb 02, 2013
Contact name Shabnam Shalapour
Organization name Charité - Universitätsmedizin Berlin
Street address Augustenburger Platz 1
City Berlin
ZIP/Postal code 13353
Country Germany
Platform ID GPL13607
Series (1)
GSE41951 Gene expression profile of the human leukemia cell line Reh after treatment with bortezomib, valproic acid or a combination of both.

Data table header descriptions
VALUE Normalized signal intensity

Data table
4 4.044902
5 26.041394
6 2.021215
7 36.782316
8 49.931563
9 0.179062
10 0.922108
11 0.042975
12 28.821124
13 75.838671
14 14.90425
15 490.027326
16 0.04313
17 2.911296
18 0.043122
19 0.043107
20 19.850361
21 10.063834
24 3.851282
25 16.657523

Total number of rows: 58717

Table truncated, full table size 864 Kbytes.

Supplementary file Size Download File type/resource
GSM1028106_US22502695_3.txt.gz 12.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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