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Status |
Public on Feb 01, 2013 |
Title |
Reh cells_valproic acid_12h |
Sample type |
RNA |
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Source name |
Reh cells, treatment with valproic acid, 12h
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Organism |
Homo sapiens |
Characteristics |
cell line: Reh tissue: leukoblasts
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Treatment protocol |
Reh cells were seeded at a denstity of 1x10E06 cells/ml in 12 well plates with corresponding drugs added from 100x stock solutions to achieve final concentrations.
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Growth protocol |
RPMI 1640 Medium, 10%FCS, 1%Pen/Strep, 37°C, 5%CO2
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Extracted molecule |
total RNA |
Extraction protocol |
mRNA was extracted by using the RNeasy Kit (Quiagen).
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Label |
cy3
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Label protocol |
Labeling was performed according to the protocol using the Agilent Low Input Quick Amp Labeling Kit (Agilent)
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Hybridization protocol |
Hybridization was performed according to the Agilent 60-mer oligo microarray processing protocol using the Agilent Gene Expression Hybridization Kit (Agilent)
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Scan protocol |
Fluorescence signals of the hybridized Agilent Microarrays were detected using Agilent’s Microarray Scanner System (Agilent).
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Data processing |
The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent). Further data analysis was performed using the Partek® Genomics Suits software, version 6.5 beta © 2009 (Partek Inc., USA).
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Submission date |
Oct 31, 2012 |
Last update date |
Feb 02, 2013 |
Contact name |
Shabnam Shalapour |
E-mail(s) |
shabnam.shalapour@charite.de
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Organization name |
Charité - Universitätsmedizin Berlin
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Street address |
Augustenburger Platz 1
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City |
Berlin |
ZIP/Postal code |
13353 |
Country |
Germany |
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Platform ID |
GPL13607 |
Series (1) |
GSE41951 |
Gene expression profile of the human leukemia cell line Reh after treatment with bortezomib, valproic acid or a combination of both. |
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