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Sample GSM1028105 Query DataSets for GSM1028105
Status Public on Feb 01, 2013
Title Reh cells_valproic acid_12h
Sample type RNA
Source name Reh cells, treatment with valproic acid, 12h
Organism Homo sapiens
Characteristics cell line: Reh
tissue: leukoblasts
Treatment protocol Reh cells were seeded at a denstity of 1x10E06 cells/ml in 12 well plates with corresponding drugs added from 100x stock solutions to achieve final concentrations.
Growth protocol RPMI 1640 Medium, 10%FCS, 1%Pen/Strep, 37°C, 5%CO2
Extracted molecule total RNA
Extraction protocol mRNA was extracted by using the RNeasy Kit (Quiagen).
Label cy3
Label protocol Labeling was performed according to the protocol using the Agilent Low Input Quick Amp Labeling Kit (Agilent)
Hybridization protocol Hybridization was performed according to the Agilent 60-mer oligo microarray processing protocol using the Agilent Gene Expression Hybridization Kit (Agilent)
Scan protocol Fluorescence signals of the hybridized Agilent Microarrays were detected using Agilent’s Microarray Scanner System (Agilent).
Data processing The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent). Further data analysis was performed using the Partek® Genomics Suits software, version 6.5 beta © 2009 (Partek Inc., USA).
Submission date Oct 31, 2012
Last update date Feb 02, 2013
Contact name Shabnam Shalapour
Organization name Charité - Universitätsmedizin Berlin
Street address Augustenburger Platz 1
City Berlin
ZIP/Postal code 13353
Country Germany
Platform ID GPL13607
Series (1)
GSE41951 Gene expression profile of the human leukemia cell line Reh after treatment with bortezomib, valproic acid or a combination of both.

Data table header descriptions
VALUE Normalized signal intensity

Data table
4 2.912614
5 15.553231
6 3.239353
7 34.88893
8 44.574282
9 0.443112
10 1.023187
11 0.031475
12 29.166037
13 44.669268
14 12.562444
15 452.477956
16 0.029513
17 2.15536
18 0.029522
19 0.029518
20 11.208825
21 7.792751
24 2.503866
25 17.043601

Total number of rows: 58717

Table truncated, full table size 863 Kbytes.

Supplementary file Size Download File type/resource
GSM1028105_US22502695_2.txt.gz 12.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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