|
| Status |
Public on Feb 04, 2015 |
| Title |
Fibros_3 |
| Sample type |
genomic |
| |
|
| Source name |
Adult skin fibroblasts
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Adult skin fibroblasts
passage: 2
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated from 5x10E6 cells using the Qiagen DNA Blood Mini Kit.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Infinium HD Methylation Assay protocol
|
| |
|
| Hybridization protocol |
Bisulfite converted DNA was amplified, fragmented and hybridized to Illumina Infinium HumanMethylation450 Beadchip using standard Infinium HD Methylation Assay protocol.
|
| Scan protocol |
Standard Infinium HD Methylation Assay protocol
|
| Description |
plastic adherent cells
|
| Data processing |
Initial analysis was performed by the Genomestudio 2010.3 (Modul M Version 1.8.5). Data were normalized with internal controls according to Illumina´s standard procedures. Methylation level at each locus was calculated with the GenomeStudio Methylation module as beta-value (ranging from 0 to 1). The number of beads per feature varies between chips and beta-values were calculated as average of at least three technical replica.
|
| |
|
| Submission date |
Oct 31, 2012 |
| Last update date |
Feb 04, 2015 |
| Contact name |
Andreas Reinisch |
| E-mail(s) |
a.reinisch@medunigraz.at
|
| Phone |
+43 31638572930
|
| Organization name |
Medical University of Graz
|
| Department |
Dept. of Internal Medicine, Division of Hematology, Stem Cell Research Unit
|
| Street address |
Auenbruggerplatz 38
|
| City |
Graz |
| ZIP/Postal code |
8036 |
| Country |
Austria |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE41933 |
Human bone and marrow niche formation by mesenchymal progenitors in vivo is predefined by an organotypic epigenetic signature |
|
