NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM102108 Query DataSets for GSM102108
Status Public on May 31, 2006
Title CD4+ CD25+_2
Sample type RNA
 
Source name CD4+CD25+
Organism Mus musculus
Characteristics genetic modification:
tissue: T-cells from spleen
Growth protocol ex vivo
Extracted molecule total RNA
Extraction protocol Qiagen RNAeasy
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at conditions recommended by the manufacturer. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GCS 3000.
Description CD4+ CD25+_2
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
 
Submission date Mar 29, 2006
Last update date May 31, 2006
Contact name Robert Geffers
E-mail(s) robert.geffers@helmholtz-hzi.de
Phone +49 531-6181-3058
Organization name HCI - Helmholtz Centre for Infection Research
Department Dep. Molecular Bacteriology
Lab Genome Analytics
Street address Inhoffenstr. 7
City Braunschweig
ZIP/Postal code 38124
Country Germany
 
Platform ID GPL339
Series (1)
GSE4571 Expression profiling of Treg cell versus their controls in human and mouse

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 410.7 P 0.002275
AFFX-BioB-M_at 521.3 P 0.000127
AFFX-BioB-3_at 307.4 P 0.001796
AFFX-BioC-5_at 2067.6 P 0.00006
AFFX-BioC-3_at 1335.7 P 0.00007
AFFX-BioDn-5_at 2930.7 P 0.000044
AFFX-BioDn-3_at 6511.8 P 0.000044
AFFX-CreX-5_at 8901.5 P 0.000044
AFFX-CreX-3_at 18779 P 0.000044
AFFX-DapX-5_at 2.8 A 0.631562
AFFX-DapX-M_at 53.4 A 0.287743
AFFX-DapX-3_at 7.5 A 0.957038
AFFX-LysX-5_at 4.3 A 0.724854
AFFX-LysX-M_at 74.7 A 0.368438
AFFX-LysX-3_at 50.8 A 0.327079
AFFX-PheX-5_at 10.4 A 0.876428
AFFX-PheX-M_at 4.9 A 0.957038
AFFX-PheX-3_at 59.2 A 0.559354
AFFX-ThrX-5_at 18.7 A 0.724854
AFFX-ThrX-M_at 13.3 A 0.559354

Total number of rows: 22690

Table truncated, full table size 612 Kbytes.




Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap